• Featured Product
  • KD/KO Validated

Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster, monkey, pig, chicken, rabbit, zebrafish, canine and More (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA

Conjugate

Unconjugated

CloneNo.

2D4H5

Cat no : 66009-1-Ig

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin



Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, HSC-T6 cells. NIH/3T3 cells, Pig brain, Rabbit brain, Rat brain, Mouse brain, Chicken brain, HEK-293 cells, HepG2 cells, K-562 cells, HHSC-T6 cells, 4T1 cells, CHO cells, mouse pancreas, rat pancreas
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.05 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66009-1-Ig targets Beta Actin in WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, hamster, monkey, pig, chicken, rabbit, zebrafish, canine samples.

Tested Reactivity human, mouse, rat, hamster, monkey, pig, chicken, rabbit, zebrafish, canine
Cited Reactivityhuman, mouse, rat, goat, chicken, Arabidopsis, Golden hamsters, Aedes albopictus, monkey, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name actin, beta
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001101
Gene Symbol ACTB
Gene ID (NCBI) 60
RRIDAB_2687938
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used "housekeeping gene" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.

 

What is the molecular weight of beta-actin?

42 kDa

 

What is the function of beta-actin?

Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).

 

What are the applications for beta-actin?

As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.

Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).

 

What are the post-transcriptional modifications (PTMs) to beta actin?

Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer's disease (PMID: 11246152). 


For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.



Protocols

Product Specific Protocols
WB protocol for Beta Actin antibody 66009-1-IgDownload protocol
IHC protocol for Beta Actin antibody 66009-1-IgDownload protocol
IF protocol for Beta Actin antibody 66009-1-IgDownload protocol
IP protocol for Beta Actin antibody 66009-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Structure of Semliki Forest virus in complex with its receptor VLDLR

Authors - Duanfang Cao
WB

Cell

Prolonged sleep deprivation induces a cytokine-storm-like syndrome in mammals

Authors - Di Sang
human,mouseWB,CoIP

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
mouseWB

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian
WB

Nature

AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence.

Authors - SangJoon Lee
WB

Nature

IL-27 signalling promotes adipocyte thermogenesis and energy expenditure.

Authors - Qian Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

MANOHAR (Verified Customer) (03-06-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: hek293, nsc cells
FH

Ziqiao (Verified Customer) (09-11-2023)

The beta actin works very well for 40ug total protein loaded

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: HEK293T cells
Beta Actin Antibody Western Blot validation (1:20000 dilution) in HEK293T cells (Cat no:66009-1-Ig)
FH

Xiaoyu (Verified Customer) (06-23-2023)

Excellent antibody, can be re-used multiple times without reduction in signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: 293T
Beta Actin Antibody Western Blot validation (1:10000 dilution) in 293T (Cat no:66009-1-Ig)
FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon
FH

Christos (Verified Customer) (04-04-2023)

Separating Gel: 10% poly-acrylamide Transfer at 400mA for 2hr onto nitrocellulose membrane at 4oC Blocking and antibodies dilutions: 5% non-fat milk in PBS-Tween20 for 1 hr at RT Incubation with primary Abs: overnight at 4oC Incubation with secondary Abs: 1hr at 4oC

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human cervix carcinoma cells/ HeLe Kyoto
Beta Actin Antibody Western Blot validation (1/1000 dilution) in Human cervix carcinoma cells/ HeLe Kyoto (Cat no:66009-1-Ig)
FH

Cynthia (Verified Customer) (03-29-2023)

Everything was fine

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Ovarian cancer cell lines
FH

Huw (Verified Customer) (01-18-2023)

Excellent antibody, can be re-used multiple times without reduction in signal

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: Fibroblasts
FH

Wu (Verified Customer) (12-05-2022)

Works good for Elisa assay

  • Applications: Other
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: hela
Beta Actin Antibody Other validation (1:250 dilution) in hela (Cat no:66009-1-Ig)
FH

Balawant (Verified Customer) (07-25-2022)

I will recommend this antibody for Immunoblotting. it is working ar 1:6000 dilution. Even it will work at 1:8000 dilution but I just used at 1:6000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:6000
  • Cell Tissue Type: colon tissue and HCT116 cells, SW620 cell
FH

Susmita (Verified Customer) (06-13-2022)

This B-Actin antibody works excellently. The dilution also significantly high. I use 1:20,000 and this works great for me.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20,000
  • Cell Tissue Type: For all invitro western blot housekeeping marker
FH

Susmita (Verified Customer) (06-13-2022)

This B-Actin antibody works excellently. The dilution also significantly high. I use 1:20,000 and this works great for me.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20,000
  • Cell Tissue Type: For all invitro western blot housekeeping marker
FH

Xin (Verified Customer) (04-17-2022)

A very good and convincing antibody to detect beta-actin in mouse tissues and various cell lines lysate.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:15000
  • Cell Tissue Type: mouse cortex lysate
Beta Actin Antibody Western Blot validation (1:15000 dilution) in mouse cortex lysate (Cat no:66009-1-Ig)
FH

Iram (Verified Customer) (04-13-2022)

Very good signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: Colon cancer cells
FH

Weihao (Verified Customer) (04-11-2022)

Very specific antibody!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000-10000
  • Cell Tissue Type: mouse BMDM
FH

Raul (Verified Customer) (03-10-2022)

Very good and strong staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SW1353 chondrosarcoma
Beta Actin Antibody Immunofluorescence validation (1:500 dilution) in SW1353 chondrosarcoma (Cat no:66009-1-Ig)
FH

Ashish (Verified Customer) (02-09-2022)

We ordered this actin antibody to detect actin as internal control in the cell lysates during the western blot, and the antibody is very sensitive.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
Beta Actin Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66009-1-Ig)
FH

James (Verified Customer) (10-07-2021)

Consistently works antibody

  • Applications: Western Blot
FH

Jinwei (Verified Customer) (10-01-2021)

The Beta Actin antibody works fine.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293
Beta Actin Antibody Western Blot validation (1:5000 dilution) in HEK293 (Cat no:66009-1-Ig)
FH

Eric (Verified Customer) (06-25-2021)

We ordered this actin antibody to detect actin in the cell lysates during the western blot, and it works very well, and the antibody is very sensitive. We highly recommend it.

  • Applications: Western Blot
  • Cell Tissue Type: human macrophages
Beta Actin Antibody Western Blot validation ( dilution) in human macrophages (Cat no:66009-1-Ig)
FH

Elisa (Verified Customer) (06-14-2021)

This antibody work really well in cells and tissues (mouse). I have also used in human skeletal muscle with good results as well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: C2C12, Heart, skeletal muscle, adipose, kidney, liver
Beta Actin Antibody Western Blot validation (1:5000 dilution) in C2C12, Heart, skeletal muscle, adipose, kidney, liver (Cat no:66009-1-Ig)
FH

Ambra (Verified Customer) (01-20-2021)

Works nicely.Used on Human brain lysates (temporal cortex).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human brain lysate
Beta Actin Antibody Western Blot validation (1:2000 dilution) in Human brain lysate (Cat no:66009-1-Ig)
FH

V (Verified Customer) (01-12-2021)

Very good product; signal is good

  • Applications: Western Blot
FH

Yu Siong (Verified Customer) (07-30-2020)

Generate very consistent result for my porcine brain endothelial cells. The antibody at dilution of 1:5000 can be reused for multiple times.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: porcine brain endothelial cells
Beta Actin Antibody Western Blot validation (1:5000 dilution) in porcine brain endothelial cells (Cat no:66009-1-Ig)
FH

Magda (Verified Customer) (12-31-2019)

I needed to run a housekeeping gene control for my protein expression test. I was testing HEK293T cell lysates. My Lab did not have a proper AB, but a colleague from a lab next door borrowed me a sample from Proteintech. I was very happy with the results, they were consistent among my triplicate. I would purchase this AB but as a HRP conjugate, as it saves more time and provides cleaner results.

  • Applications: Western Blot,
  • Cell Tissue Type: HEK293T
FH

Elisa (Verified Customer) (10-21-2019)

The antibody works very nicely, very clean and I used only 10 uL of protein.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Cell line C2C12
FH

Tracy Chih-Ting (Verified Customer) (09-24-2019)

I used this antibody for Hydra actin immunostaining. The outcome is quite good.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
FH

Rui (Verified Customer) (09-02-2019)

Very specific and strong labeling in whole cell lysates.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Cos-7
FH

Huai-Chin (Verified Customer) (06-19-2019)

Very good anitbody. Works well in Western for various type of cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Primary cells, various cell lines
FH

Francesco (Verified Customer) (06-12-2019)

in the testing staining it kind of worked, even if with higher dilution it could work better.anyway further test should be done to confirm it.if results are reproducible it might be a valid alternative to more expensive antibody

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/1500
  • Cell Tissue Type: Brain cells
FH

Kyosuke (Verified Customer) (06-05-2019)

This antibody works very well for Western blot using vascular tissue or cell lysates.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: aorta