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  • KD/KO Validated

SEC61G Polyclonal antibody

SEC61G Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11147-2-AP

Synonyms

Sec61 gamma subunit, SEC61G, SSS1



Tested Applications

Positive WB detected inEC109 cells, HEK-293 cells, DC2.4 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11147-2-AP targets SEC61G in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC61G fusion protein Ag1624
Full Name Sec61 gamma subunit
Calculated Molecular Weight 7.7 kDa
Observed Molecular Weight 7.7 kDa
GenBank Accession NumberBC009480
Gene Symbol SEC61G
Gene ID (NCBI) 23480
RRIDAB_2254450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC61G is a subunit of the SEC61 complex, which also contains alpha (SEC61A) and beta (SEC61B) subunits. The SEC61 complex is the central component of the protein translocation apparatus on the endoplasmic reticulum membrane. The gene of SEC61G maps to chromosome 7p11.2, and encodes a 68-amino acid single-pass membrane protein. This antibody can recognize endogenous SEC61G that migrates at a molecular mass of ~8 kDa.

Protocols

Product Specific Protocols
WB protocol for SEC61G antibody 11147-2-APDownload protocol
IHC protocol for SEC61G antibody 11147-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Immunity

The translocon protein sec61 mediates antigen transport from endosomes in the cytosol for cross-presentation to CD8(+) T cells.

Authors - Matthias Zehner
  • KD Validated
mouseWB

Proc Natl Acad Sci U S A

Ablation of cholesterol biosynthesis in neural stem cells increases their VEGF expression and angiogenesis but causes neuron apoptosis.

Authors - Saito Kanako K
humanWB

Proc Natl Acad Sci U S A

A scalable strategy for high-throughput GFP tagging of endogenous human proteins.

Authors - Manuel D Leonetti
humanWB

Cancer Res

Glioblastoma proto-oncogene SEC61gamma is required for tumor cell survival and response to endoplasmic reticulum stress.

Authors - Lu Zheming Z
  • KD Validated
humanWB

Elife

An allosteric Sec61 inhibitor traps nascent transmembrane helices at the lateral gate.

Authors - Andrew L Mackinnon
humanIHC

J Pathol

Genome-wide analysis of DNA copy number alterations and gene expression in gastric cancer.

Authors - Tsukamoto Y Y