SLC24A6 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC24A6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 21430-1-AP

Synonyms

NCKX6, NCLX, SLC24A6, SLC8B1

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Validation Data Gallery

• 

  WB analysis using 21430-1-AP

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21430-1-AP (SLC24A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21430-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21430-1-AP (SLC24A6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

21430-1-AP targets SLC24A6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC24A6 fusion protein Ag16034
• Full Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 6
• Calculated Molecular Weight: 584 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC098360
• Gene Symbol: SLC24A6
• Gene ID (NCBI): 80024
• RRID: AB_10858637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC24A6, also named as NCLX, is a kind of mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion by mediating the exchange of 3 sodium ions per 1 calcium ion. It has been reported that SLC24A6 plays a key role in attenuating potential ROS production and injury upon cardiac reperfusion. SLC24A6 is associated with mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion. (PMID: 32728214, 20018762, 28219928)

Publications

Species	Application	Title
mouse	WB	Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
mouse	WB	J Cardiovasc Transl Res; CD137 Signal Mediates Cardiac Ischemia-Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes.; Authors - Yao Xu
human	WB	Oncol Lett; Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX; Authors - Jiang Chang
rat	WB,IF	Cell Biochem Biophys; Astragalus Polysaccharides Reduce High-glucose-induced Rat Aortic Endothelial Cell Senescence and Inflammasome Activation by Modulating the Mitochondrial Na+/Ca2+ Exchanger.; Authors - Xin-Yu Miao
rat	WB, IF	J Geriatr Cardiol; The mitochondrial Na(+)/Ca(2+) exchanger may reduce high glucose-induced oxidative stress and nucleotide-binding oligomerization domain receptor 3 inflammasome activation in endothelial cells.; Authors - Yuan Zu ; KD Validated
human	IHC,IF	Cell Death Dis; Lon upregulation contributes to cisplatin resistance by triggering NCLX-mediated mitochondrial Ca2+ release in cancer cells.; Authors - Vidhya Tangeda