Septin 2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Septin 2 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F3B2

Cat no : 60075-1-PBS

Synonyms

DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, SEPT2, SEPT2/septin 2, Septin 2, septin2

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Validation Data Gallery

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  WB analysis using 60075-1-Ig (same clone as 60075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60075-1-Ig (same clone as 60075-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at various dilutions incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60075-1-Ig (same clone as 60075-1-PBS)

  WB result of Septin 2 antibody (60075-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60075-1-Ig (same clone as 60075-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60075-1-Ig (same clone as 60075-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60075-1-Ig (same clone as 60075-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60075-1-Ig (same clone as 60075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 60075-1-Ig (same clone as 60075-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 60075-1-Ig (same clone as 60075-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60075-1-Ig (Septin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60075-1-Ig (Septin 2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60075-1-Ig(Septin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE cells using 60075-1-Ig (same clone as 60075-1-PBS)

  IF result of anti-Septin 2 (60075-1-Ig, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 2. Blue: DNA. 1% PFA fixed. This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Septin 2 antibody (60075-1-Ig, Clone: 2F3B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 60075-1-Ig (same clone as 60075-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (60075-1-Ig, Clone:2F3B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60075-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60075-1-PBS targets Septin 2 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Septin 2 fusion protein Ag1946
• Full Name: septin 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC014455
• Gene Symbol: SEPT2
• Gene ID (NCBI): 4735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)