Syntaxin 6 Monoclonal antibody, PBS Only

Syntaxin 6 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B6G4

Cat no : 60059-1-PBS

Synonyms

Cell and organelle markers, Golgi Marker, STX6, syntaxin 6

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Validation Data Gallery

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  WB analysis using 60059-1-Ig (same clone as 60059-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60059-1-Ig (same clone as 60059-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 60059-1-Ig (same clone as 60059-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 60059-1-Ig (same clone as 60059-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Syntaxin 6 antibody (60059-1-Ig, Clone: 2B6G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60059-1-PBS targets Syntaxin 6 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Syntaxin 6 fusion protein Ag1284
• Full Name: syntaxin 6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC009944
• Gene Symbol: STX6
• Gene ID (NCBI): 10228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.