CoraLite® Plus 488-conjugated TPM4 Monoclonal antibody

• Featured Product
• KD/KO Validated

TPM4 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E12D12

Cat no : CL488-67244

Synonyms

TM30p1, TPM4, tropomyosin 4, Tropomyosin alpha 4 chain

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Validation Data Gallery

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  WB analysis using CL488-67244

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67244 (TPM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat skeletal muscle tissue, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67244 targets TPM4 in WB applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TPM4 fusion protein Ag6947
• Full Name: tropomyosin 4
• Calculated Molecular Weight: 248 aa, 29 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC037576
• Gene Symbol: TPM4
• Gene ID (NCBI): 7171
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.