CoraLite® Plus 488-conjugated TrkC Monoclonal antibody

TrkC Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A7F11

Cat no : CL488-66380

Synonyms

GP145 TrkC, gp145(trkC), NT 3 growth factor receptor, NTRK3, Trk C, TRKC, TrkC tyrosine kinase

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Validation Data Gallery

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  FC experiment of SH-SY5Y using CL488-66380

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TrkC (CL488-66380, Clone:2A7F11) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66380 targets TrkC in FC (Intra) applications and shows reactivity with human, rat, mouse, pig samples.

• Tested Reactivity: human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TrkC fusion protein Ag2635
• Full Name: neurotrophic tyrosine kinase, receptor, type 3
• Calculated Molecular Weight: 839 aa, 94 kDa
• Observed Molecular Weight: 110 kDa, 160 kDa
• GenBank Accession Number: BC013693
• Gene Symbol: NTRK3
• Gene ID (NCBI): 4916
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.