beta Tubulin Antibody 5 Publications

Mouse Monoclonal| Catalog number: 66240-1-Ig |CloneNo.: 1D4A4

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Freight/Packing

Con: 214 μg/150 μl

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Species specificity:
human, mouse, rat, nematode, pig, zebrafish

Positive WB detected in:
RAW 264.7 cells

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human brain tissue, human brain tissue, human breast cancer tissue, rat brain tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:5000-1:100000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:1000
IF : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2a

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
tubulin, beta 3

Calculated molecular weight:
450aa,50 kDa

Observed molecular weight:
50-55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TUBB3

Synonyms
beta tubulin, MC1R, TUBB, Tubulin-beta
Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.


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