CoraLite®594-conjugated p120 Catenin Monoclonal antibody

• Featured Product
• KD/KO Validated

p120 Catenin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F7H8

Cat no : CL594-66208

Synonyms

CAS, Catenin delta 1, CTNND, CTNND1, KIAA0384, p120, p120 catenin, p120(cas), p120(ctn), P120CAS, P120CTN

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-66208

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using the CoraLite®594-conjugated version of this antibody, CL594-66208 (p120 Catenin antibody), at dilution of 1:100.

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  IF Staining of human breast cancer using CL594-66208

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using the CoraLite®594-conjugated version of this antibody, CL594-66208 (p120 Catenin antibody), at dilution of 1:100.

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  IF Staining of MCF-7 using CL594-66208

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL594-66208 (p120 Catenin antibody) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: MCF-7 cells, human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66208 targets p120 Catenin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: p120 Catenin fusion protein Ag2824
• Full Name: catenin (cadherin-associated protein), delta 1
• Calculated Molecular Weight: 948 aa, 105 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: BC010501
• Gene Symbol: p120 Catenin
• Gene ID (NCBI): 1500
• RRID: AB_2883536
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).