Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18138-1-AP targets ABCD1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12757 Product name: Recombinant human ABCD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 358-745 aa of BC025358 Sequence: SESDAEAVKKAALEKKEEELVSERTEAFTIARNLLTAAADAIERIMSSYKEVTELAGYTARVHEMFQVFEDVQRCHFKRPRELEDAQAGSGTIGRSGVRVEGPLKIRGQVVDVEQGIICENIPIVTPSGEVVVASLNIRVEEGMHLLITGPNGCGKSSLFRILGGLWPTYGGVLYKPPPQRMFYIPQRPYMSVGSLRDQVIYPDSVEDMQRKGYSEQDLEAILDVVHLHHILQREGGWEAMCDWKDVLSGGEKQRIGMARMFYHRPKYALLDECTSAVSIDVEGKIFQAAKDAGIALLSITHRPSLWKYHTHLLQFDGEGGWKFEKLDSAARLSLTEEKQRLEQQLAGIPKMQRRLQELCQILGEAVAPAHVPAPSPQGPGGLQGAST Predict reactive species |
| Full Name | ATP-binding cassette, sub-family D (ALD), member 1 |
| Calculated Molecular Weight | 745 aa, 83 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC025358 |
| Gene Symbol | ABCD1 |
| Gene ID (NCBI) | 215 |
| RRID | AB_2273333 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P33897 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ABCD1 antibody 18138-1-AP | Download protocol |
| IF protocol for ABCD1 antibody 18138-1-AP | Download protocol |
| IHC protocol for ABCD1 antibody 18138-1-AP | Download protocol |
| IP protocol for ABCD1 antibody 18138-1-AP | Download protocol |
| WB protocol for ABCD1 antibody 18138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ABCD1 Polyclonal antibody (Cat# 18138-1-AP) has 4 citations published in Signal Transduction and Targeted Therapy, Leukemia, EMBO Journal, and International Endodontic Journal. The associated research fields include peroxisomal ABC transporter biology, multiple myeloma signaling, ciliary cholesterol metabolism in Zellweger syndrome, and mitochondrial function in dental stem cells. All citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP | ||
EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
| ||
Int Endod J Mitochondrial Function Is Required to Preserve Viability of Stem Cells From the Apical Papilla Following Porphyromonas endodontalis Infection. |
