IHCeasy PSMB2 Ready-To-Use IHC Kit
PSMB2 Ready-to-use reagent kit for IHC.
Cat no : KHC1615
Synonyms
20S proteasome, HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2
Validation Data Gallery
Product Information
KHC1615 is a ready-to-use IHC kit for staining of PSMB2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy PSMB2 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
PSMB2, Proteasome subunit beta type-2, is also known as 20S proteasome subunit beta-4. The proteasome is responsible for degradation of short lived and misfolded cytosolic and nuclear proteins in the cell and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMB2 is up-regulated in ovarian cancer cell lines. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD (vitamin A depletion) brain regions. Knockdown of PSMB2 suppresses hepatocellular carcinoma and gastric cancer cell proliferation and invasion.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | 20S proteasome, HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2 |