LYPLA1 Polyclonal antibody

LYPLA1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16055-1-AP

Synonyms

Acyl protein thioesterase 1, APT 1, APT1, hAPT1, LPL I, LPL1, LYPLA1, Lysophospholipase 1, lysophospholipase I, LYSOPLA, LysoPLA I

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Validation Data Gallery

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  WB analysis using 16055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 16055-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16055-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16055-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16055-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16055-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16055-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16055-1-AP

  IP result of anti-LYPLA1 (IP:16055-1-AP, 4ug; Detection:16055-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 16055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16055-1-AP (LYPLA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16055-1-AP (LYPLA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16055-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16055-1-AP (LYPLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, human brain tissue, human liver tissue, rat liver tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below

Product Information

16055-1-AP targets LYPLA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LYPLA1 fusion protein Ag9022
• Full Name: lysophospholipase I
• Calculated Molecular Weight: 230 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010397
• Gene Symbol: LYPLA1
• Gene ID (NCBI): 10434
• RRID: AB_2234851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYPLA1(Lysophospholipase 1) is also named as APT1(Acyl-protein thioesterase 1), LPL1 and belongs to the AB hydrolase 2 family. It is an important enzyme responsible for depalmitoylation of palmitoyl proteins and works as a thioesterase that cleaves the S-palmitoyl group of H-Ras, heterotrimeric G protein alfa subunit (Ga) , regulator of G protein signaling 4 (RGS4) and the endothelial isoform of nitric oxide synthase (eNOS) in vitro(PMID:19439193).

Publications

Species	Application	Title
human	IHC	J Transl Med; Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment; Authors - Guohong Liu
human	WB	Ecotoxicol Environ Saf; PM2.5 promoted lipid accumulation in macrophage via inhibiting JAK2/STAT3 signaling pathways and aggravating the inflammatory reaction.; Authors - Liwei Yang
human	WB	PLoS One; Development of an activity-based probe for acyl-protein thioesterases.; Authors - Megan Garland
human	IHC	Drug Metab Dispos; Identification of Bioactivating Enzymes Involved in the Hydrolysis of Laninamivir Octanoate, a Long-acting Neuraminidase Inhibitor, in Human Pulmonary Tissue.; Authors - Kumiko Koyama
human	WB	Autophagy; Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1; Authors - Ying Liu
human	IHC,WB	Reprod Sci; Estrogen Regulates Scribble Localization in Endometrial Epithelial Cells Through Acyl Protein Thioesterase (APT)-Mediated S-Palmitoylation in Adenomyosis; Authors - Zhixing Jin