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PHACS Polyclonal antibody

PHACS Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11491-1-AP

Synonyms

ACC synthase like protein 1, ACCS, ACS, PHACS

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using PHACS Polyclonal antibody (11491-1-AP)

    WB analysis of MDA-MB-453s using 11491-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PHACS Polyclonal antibody (11491-1-AP)

    WB analysis of mouse testis using 11491-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PHACS Polyclonal antibody (11491-1-AP)

    WB analysis of mouse testis using 11491-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PHACS Polyclonal antibody (11491-1-AP)

    WB analysis of HeLa using 11491-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHACS Polyclonal antibody (11491-1-AP)

    IHC staining of human colon cancer using 11491-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11491-1-AP (PHACS antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inMDA-MB-453s cells, HeLa cells, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11491-1-AP targets PHACS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHACS fusion protein Ag2056
Full Name 1-aminocyclopropane-1-carboxylate synthase homolog (Arabidopsis)(non-functional)
Calculated Molecular Weight 501 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC020197
Gene Symbol ACCS
Gene ID (NCBI) 84680
RRIDAB_2222681
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACCS(1-aminocyclopropane-1-carboxylate synthase-like protein 1) is also named as PHACS and belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. The deduced 501-amino acid protein has a calculated molecular mass of 58 kD. It contains overlapping aminotransferase I and beta-eliminating lyase domains, and it shares structural similarity with aspartate aminotransferase, tyrosine aminotransferase, and enzymes that catalyze beta-elimination reactions on amino acids.

Protocols

Product Specific Protocols
WB protocol for PHACS antibody 11491-1-APDownload protocol
IHC protocol for PHACS antibody 11491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols