Spot Capture and
Detection System

For immunoprecipitation, protein purification & immunofluorescence

ChromoTek’s proprietary and novel Spot-Tag system is the first peptide-tag specific Nanobody for universal capture & detection applications. It comprises the Spot-Tag®, an inert 12 amino acid peptide-tag (PDRVRAVSHWSS), and Spot-Nanobodies that specifically bind to Spot-tagged proteins with high affinity.

Applications & Tools:

Explore Spot Capture and Detection Tools
Interaction of Spot-Nanobody (green) with Spot Peptide

Interaction of Spot-Nanobody (green) with Spot Peptide.

For more details about the Spot-Tag system, click here.

For ease of cloning, we offer a range of Spot vectors:

Spot-Tag Vectors for cloning
Spot-Tag Vectors - positive control

Explore the range

 

For additional cloning strategies see below suggestions for codon-optimized sequences and linkers:

Amino acid sequence Spot-Tag
PDRVRAVSHWSS

Position Spot-Tag
N-terminus and C-terminus

Codon optimized sequences for Spot-Tag
E. coli
CCG GAT CGC GTG CGC GCA GTC TCT CAC TGG AGC AGC

D. melanogaster
CCC GAT CGC GTG CGT GCC GTG AGC CAC TGG AGC TCG

S. cerevisiae
CCA GAT AGA GTT AGA GCT GTT TCT CAT TGG TCT TCT

Human cell line
CCA GAC CGC GTG CGC GCC GTG AGC CAT TGG AGC AGC

A. thaliana
CCT GAT AGA GTT AGA GCT GTT TCT CAT TGG TCT TCT

Linker (optional)
We recommend a short Gly-Ser linker if there are adjacent acidic amino acids (Glu/Asp):
E. coli  GGT TCT
S. cerevisiae   GGT TCT
Human cell line   GGC AGC

Product Formats (Product citations in parentheses)
Spot-Cap Agarose beads
Spot-Label® ATTO488ATTO594,   Alexa Fluor® 488Alexa Fluor® 568Alexa Fluor® 647
Spot-Trap® Agarose (3)Magnetic Agarose (6)Magnetic Particles M-270
Spot VHH Unconjugated
Spot-tag® antibody (28A5) Unconjugated
Spot-Tag Vectors for cloning    ev-1, ev-2, ev-3, ev-4, ev-5, ev-6, ev-7, ev-8
Spot Vectors - positive controls     ev-31, ev-32, ev-33