Spot Capture and
Detection System
For immunoprecipitation, protein purification & immunofluorescence
ChromoTek’s proprietary and novel Spot-System is the first peptide-tag specific Nanobody for universal capture & detection applications. It comprises the Spot-Tag®, an inert 12 amino acid peptide-tag (PDRVRAVSHWSS), and Spot-Nanobodies that specifically bind to Spot-tagged proteins with high affinity.
Applications & Tools:
- Immunoprecipitation (IP) / Co-IP: Spot-Trap®
- Co-IP/MS: Spot-Trap and iST Spot-Trap Kit
- Protein purification: Spot-Cap®
- Immunofluorescence: Spot-Label®
- Western Blotting: Spot-tag antibody [28a5]
Applications:
- Live-cell imaging of endogenous proteins
- High content analysis of cells, tissues, whole organs (sections), and organisms (sections)
- Investigation of small molecules in secondary screens – either as biomarkers or controls
| Product | Formats (Product citations in parentheses) |
|---|---|
| Spot-Cap | Agarose beads |
| Spot-Label® | ATTO488, ATTO594, Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647 |
| Spot-Trap® | Agarose (3), Magnetic Agarose (6), Magnetic Particles M-270 |
| Spot VHH | Unconjugated |
| Spot-tag® antibody (28A5) | Unconjugated |