ChromoTek Nano-Traps

The gold standard in immunoprecipitation

Nano-Traps are the ready-to-use affinity beads for IP that allow for fast and reliable, single-band pulldowns of even low abundance proteins.

  • No contaminating heavy & light antibody chains or binding proteins​
  • Short Incubation Time (30-60 mins)
  • High affinity pulldowns of even low abundance proteins​
  • Lowest background in class​
  • Stable under stringent washing conditions ​
  • Recombinant Expression ​
  • No contaminating heavy & light antibody chains or binding proteins​
  • High affinity pulldowns of even low abundance proteins​
  • Stable under stringent washing conditions ​
  • Short Incubation Time (30-60 mins)​
  • Lowest background in class​
  • Recombinant Expression ​

Low background, no extra bands & high specificity will improve your pulldown assay significantly.

Immunoprecipitation (IP) of a protein of interest (POI) using Nano-Traps (left) compared with a conventional antibodies coupled to Protein A/G beads (right). When using Nano-Traps, the amount of immunoprecipitated POI is higher and the background is reduced in comparison to an IP conducted with the conventional antibody. Nano-Traps also provide pure fractions of immunoprecipitated POI without contamination of heavy and light antibody chains.

GFP Trap Vs Ab Gel Image

Nano-Traps are highly stable in contrast to conventional antibodies. Once bound to their target proteins, very stringent washing conditions can be applied to remove any unwanted proteins as well as reduce background. Additionally, Nano-Traps can also be used in the presence of virtually any lysis buffer, such more specialized ones needed for ubiquitination assays or phosphorylation studies.​

GFP Trap Buffer Compatibility

Analysis of GFP-Trap buffer compatibility. The GFP-Trap is compatible with common wash and lysis buffers, including those with high concentrations of NaCl, NP-40, SDS, and Urea. No matter the wash condition, GFP-tagged proteins remain bound to trap, which no protein remaining in the flow-through fraction.

GFP Trap Vs Antibody Workflow

Nano-Traps are ready-to-use, with the nanobody pre-conjugated to beads. This significantly speeds up the IP workflow with no pre-incubation necessary and pulldowns completed within 60 minutes

GFP Trap Time Course Experiment

Time-course pulldown of GFP-tagged proteins using GFP-Trap. Most tagged-proteins are bound by the trap within 5 minutes with all protein being captured by 30 mins.

V5 Trap Background Comparison

IP from different cell lysates without V5-tagged proteins using V5-Trap. The V5-Trap has remarkably low background with no non-specific binding seen in the bound fraction. ​

HA Trap Comparison To Competitor

Pulldown of TOM70-HA fusion protein from transfected HEK293T cells using either HA-Trap (left) or a competitor resin (right). Pulldowns with HA-Trap result in far cleaner, single-band pulldowns with significantly less artifact binding compared to the competitor product.​

There is a built-in discount when scaling up to our 200- and 400- reaction sizes. You can save nearly 25% when purchasing in bulk. Our recombinant expression system ensures the supply of even larger quantities upon request.

GFP Trap Vs Ab Gel Image