Secondary Antibodies

Proteintech offers a comprehensive portfolio of highly sensitive secondary antibodies which have been validated to detect unconjugated primary antibodies.

These secondary antibodies are conjugated to various fluorescent dyes and enzymes suited for all your experimental needs. These products are tested to prove its performance in intended applications like Western blot, Immunofluorescence, Immunohistochemistry, Flow cytometry and ELISA.
ChromoTek’s Nano-Secondary® reagents use a novel class of secondary antibodies for higher resolution & cleaner images. These reagents consist of Nanobodies/ VHHs that bind to primary antibodies with high affinity in a species and subtype specific manner. Nano-Secondaries reagents have secondary antibodies conjugated to Alexa Fluor® dyes.

Select your secondaries based on conjugates

Nano-Secondary reagents

  • One-step staining
  • Recombinant
  • Better resolution

Nano-Secondary reagents use a novel class of secondary antibodies and have certain advantages over the conventional secondaries.

  • Pre-incubation or parallel incubation of Nano-Secondary reagents with primary antibodies, i.e., one-step incubation, reduce incubation times and enhance multiplexing capabilities.
  • The recombinant process of developing Nano-Secondary reagents ensures high lot to lot consistency.
  • Smaller size of Nano-Secondary reagents has been proven to enhance image resolution in Super resolution Microscopy.

Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondary reagents and one anti-rabbit Nano-Secondary reagent. Green: mouse IgG1 anti-COX4 + Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104]. Magenta: mouse IgG2b anti-Tubulin + Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 647 [CTK0105, CTK0106]. Yellow: rabbit anti-Lamin + Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 [CTK0101, CTK0102]. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Featured Products

Recent Publications

Acta Biochim Biophys Sin (Shanghai)

Effect of Sox9 on TGF-β1-mediated atrial fibrosis

Hechuan Wang


Secondary antibody selection

How to choose the best secondary antibody for your application

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Spectra viewer

Design and plan your experiments using our spectra viewer

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CoraLite® fluorescent dye-conjugated antibodies

CoraLite dyes have equivalent brightness to the Alexa Fluor® dyes.

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