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Secondary Antibodies

Proteintech offers a comprehensive portfolio of highly sensitive secondary antibodies which have been validated to detect unconjugated primary antibodies in various applications. Our secondary antibodies are offered unconjugated or conjugated to HRP, Alkaline phosphatase, Biotin, and a range of fluorescent dyes including our high performing CoraLite® fluorescent dyes.

Western blot

Product Name
Catalog No.
Size
Applications
HRP Secondary Antibodies      
Streptavidin, HRP conjugate SA00001-0 200ul* ELISA, WB
Goat anti-mouse IgG (H+L), HRP conjugate SA00001-1 200ul* ELISA, WB, IHC/ICC
Affinipure Goat Anti-Guinea pig IgG(H+L), HRP conjugate SA00001-12 200ul* ELISA, WB
Affinipure Goat Anti-Horse IgG(H+L), HRP conjugate SA00001-13 200ul* ELISA, WB
Goat anti-rat IgG (H+L), HRP conjugate SA00001-15 200ul* ELISA, WB, IHC/ICC
Goat anti-human IgG (H+L), HRP conjugate SA00001-17 200ul* ELISA, WB, IHC/ICC
Protein A from S.aureus, HRP conjugate SA00001-18 200ul* IP, WB
Goat anti-rabbit IgG (H+L), HRP conjugate SA00001-2 200ul* ELISA, WB, IHC/ICC
Rabbit anti-goat IgG (H+L), HRP conjugate SA00001-4 200ul* ELISA, WB, IHC/ICC
Affinipure Goat Anti-Swine IgG(H+L), HRP conjugate SA00001-5 200ul* ELISA, WB
Mouse Anti-Rabbit IgG Light Chain Specific, HRP conjugate SA00001-7L 200ul* ELISA, WB
Mouse anti-rabbit IgG (Heavy Chain), HRP conjugate HRP-66467 100ul ELISA, WB
       
AP Secondary Antibodies      
Goat anti-mouse IgG (H+L), AP conjugate SA00002-1 200ul* ELISA, WB
Goat anti-rabbit IgG (H+L), AP conjugate SA00002-2 200ul* ELISA, WB
Goat anti-human IgG (H+L), AP conjugate SA00002-8 200ul* ELISA, WB
Goat anti-rat IgG (H+L), AP conjugate SA00002-9 200ul* ELISA, WB
       
Biotin Secondary Antibodies      
Goat anti-mouse IgG (H+L), biotin conjugate SA00004-1 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
Rabbit anti-goat IgG (H+L), biotin conjugate SA00004-4 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
       
Unconjugated Secondary Antibodies      
Mouse anti-rabbit IgG (Heavy Chain)，unconjugated 66467-1-Ig 150ul WB, IF， IHC，ELISA

* In Europe, only 100ul vials are available.

Immunofluorescence

Product Name
Catalog No.
Size
Applications
CoraLite Secondary Antibodies      
Affinipure Goat Anti-Rabbit IgG(H+L), CoraLite488 conjugate  SA00013-2 200ul* IF, Flow cyt
Mouse anti-rabbit IgG (Heavy Chain), CL594 conjugate  CL594-66467 100ul IF 
       
FITC Secondary Antibodies      
Goat anti-mouse IgG (H+L), FITC conjugate SA00003-1 200ul* Flow Cyt, ICC/IF, IHC
Goat anti-rat IgG (H+L), FITC conjugate SA00003-11 200ul* IF, Flow cyt
Goat anti-human IgG (H+L), FITC conjugate SA00003-12 200ul* IF, Flow cyt
Goat anti-rabbit IgG (H+L), FITC conjugate SA00003-2 200ul* Flow Cyt, ICC/IF, IHC
Rabbit anti-goat IgG (H+L), FITC conjugate SA00003-4 200ul* IF, Flow cyt
 
Biotin Secondary Antibodies      
Goat anti-mouse IgG (H+L), biotin conjugate SA00004-1 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
Rabbit anti-goat IgG (H+L), biotin conjugate SA00004-4 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
 
TRITC Secondary Antibodies      
Goat anti-mouse IgG (H+L), TRITC conjugate SA00007-1 200ul* Flow Cyt, ICC/IF, IHC
Goat anti-human IgG (H+L), TRITC conjugate SA00007-10 200ul* IF, Flow cyt
Goat anti-rabbit IgG (H+L), TRITC conjugate SA00007-2 200ul* Flow Cyt, ICC/IF, IHC
Rabbit anti-goat IgG (H+L), TRITC conjugate SA00007-4 200ul* IF, Flow cyt
 
R-PE Secondary Antibodies      
Goat anti-rabbit IgG (H+L), R-PE conjugate SA00008-2 200ul* IF, Flow cyt
Donkey anti-goat IgG (H+L), R-PE conjugate SA00008-3 200ul* IF, Flow cyt
 
Cy3 Secondary Antibodies      
Goat anti-mouse IgG (H+L), Cy3 conjugate SA00009-1 200ul* IF, Flow cyt
Goat anti-rabbit IgG (H+L), Cy3 conjugate SA00009-2 200ul* IF, Flow cyt
Rabbit anti-goat IgG (H+L), Cy3 conjugate SA00009-4 200ul* IF, Flow cyt
 
Unconjugated Secondary Antibodies      
Mouse anti-rabbit IgG (Heavy Chain)，unconjugated 66467-1-Ig 150ul WB, IF， IHC，ELISA

* In Europe, only 100ul vials are available.

Immunohistochemistry

Product Name
Catalog No.
Size
Applications
HRP Secondary Antibodies       
Goat anti-mouse IgG (H+L), HRP conjugate SA00001-1 200ul* ELISA, WB, IHC/ICC
Goat anti-rat IgG (H+L), HRP conjugate SA00001-15 200ul* ELISA, WB, IHC/ICC
Goat anti-human IgG (H+L), HRP conjugate SA00001-17 200ul* ELISA, WB, IHC/ICC
Goat anti-rabbit IgG (H+L), HRP conjugate SA00001-2 200ul* ELISA, WB, IHC/ICC
Rabbit anti-goat IgG (H+L), HRP conjugate SA00001-4 200ul* ELISA, WB, IHC/ICC
       
FITC Secondary Antibodies      
Goat anti-mouse IgG (H+L), FITC conjugate SA00003-1 200ul* Flow Cyt, ICC/IF, IHC
Goat anti-rabbit IgG (H+L), FITC conjugate SA00003-2 200ul* Flow Cyt, ICC/IF, IHC
       
Biotin Secondary Antibodies      
Goat anti-mouse IgG (H+L), biotin conjugate SA00004-1 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
Rabbit anti-goat IgG (H+L), biotin conjugate SA00004-4 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
       
TRITC Secondary Antibodies      
Goat anti-mouse IgG (H+L), TRITC conjugate SA00007-1 200ul* Flow Cyt, ICC/IF, IHC
Goat anti-rabbit IgG (H+L), TRITC conjugate SA00007-2 200ul* Flow Cyt, ICC/IF, IHC
       
Unconjugated Secondary Antibodies      
Mouse anti-rabbit IgG (Heavy Chain)，unconjugated 66467-1-Ig 150ul WB, IF， IHC，ELISA

* In Europe, only 100ul vials are available.

ELISA

Product Name
Catalog No.
Size
Applications
HRP Secondary Antibodies      
Streptavidin, HRP conjugate SA00001-0 200ul* ELISA, WB
Goat anti-mouse IgG (H+L), HRP conjugate SA00001-1 200ul* ELISA, WB, IHC/ICC
Affinipure Goat Anti-Guinea pig IgG(H+L), HRP conjugate SA00001-12 200ul* ELISA, WB
Affinipure Goat Anti-Horse IgG(H+L), HRP conjugate SA00001-13 200ul* ELISA, WB
Goat anti-rat IgG (H+L), HRP conjugate SA00001-15 200ul* ELISA, WB, IHC/ICC
Goat anti-human IgG (H+L), HRP conjugate SA00001-17 200ul* ELISA, WB, IHC/ICC
Goat anti-rabbit IgG (H+L), HRP conjugate SA00001-2 200ul* ELISA, WB, IHC/ICC
Rabbit anti-goat IgG (H+L), HRP conjugate SA00001-4 200ul* ELISA, WB, IHC/ICC
Affinipure Goat Anti-Swine IgG(H+L), HRP conjugate SA00001-5 200ul* ELISA, WB
HRP-Mouse Anti-Rabbit IgG Light Chain Specific SA00001-7L 200ul* ELISA, WB
Mouse anti-rabbit IgG (Heavy Chain), HRP conjugate HRP-66467 100ul ELISA, WB
       
AP Secondary Antibodies      
Goat anti-mouse IgG (H+L), AP conjugate SA00002-1 200ul* ELISA, WB
Goat anti-rabbit IgG (H+L), AP conjugate SA00002-2 200ul* ELISA, WB
Goat anti-human IgG (H+L), AP conjugate SA00002-8 200ul* ELISA, WB
Goat anti-rat IgG (H+L), AP conjugate SA00002-9 200ul* ELISA, WB
       
Biotin Secondary Antibodies      
Goat anti-mouse IgG (H+L), biotin conjugate SA00004-1 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
Rabbit anti-goat IgG (H+L), biotin conjugate SA00004-4 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
       
Unconjugated Secondary Antibodies      
Mouse anti-rabbit IgG (Heavy Chain)，unconjugated 66467-1-Ig 150ul WB, IF， IHC，ELISA

* In Europe, only 100ul vials are available.

Flow Cytometry

Product Name
Catalog No.
Size
Applications
CoraLite Secondary Antibodies      
Affinipure Goat Anti-Rabbit IgG(H+L), CoraLite488 conjugate SA00013-2 200ul* IF, Flow cyt
       
FITC Secondary Antibodies      
Goat anti-mouse IgG (H+L), FITC conjugate SA00003-1 200ul* Flow Cyt, ICC/IF, IHC
Goat anti-rat IgG (H+L), FITC conjugate SA00003-11 200ul* IF, Flow cyt
Goat anti-human IgG (H+L), FITC conjugate SA00003-12 200ul* IF, Flow cyt
Goat anti-rabbit IgG (H+L), FITC conjugate SA00003-2 200ul* Flow Cyt, ICC/IF, IHC
Rabbit anti-goat IgG (H+L), FITC conjugate SA00003-4 200ul* IF, Flow cyt
       
Biotin Secondary Antibodies      
Goat anti-mouse IgG (H+L), biotin conjugate SA00004-1 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
Rabbit anti-goat IgG (H+L), biotin conjugate SA00004-4 200ul* ELISA, WB, IHC/ICC, IF, Flow Cyt
       
TRITC Secondary Antibodies      
Goat anti-mouse IgG (H+L), TRITC conjugate SA00007-1 200ul* Flow Cyt, ICC/IF, IHC
Goat anti-human IgG (H+L), TRITC conjugate SA00007-10 200ul* IF, Flow cyt
Goat anti-rabbit IgG (H+L), TRITC conjugate SA00007-2 200ul* Flow Cyt, ICC/IF, IHC
Rabbit anti-goat IgG (H+L), TRITC conjugate SA00007-4 200ul* IF, Flow cyt
       
R-PE Secondary Antibodies      
Goat anti-rabbit IgG (H+L), R-PE conjugate SA00008-2 200ul* IF, Flow cyt
Donkey anti-goat IgG (H+L), R-PE conjugate SA00008-3 200ul* IF, Flow cyt
       
Cy3 Secondary Antibodies      
Goat anti-mouse IgG (H+L), Cy3 conjugate SA00009-1 200ul* IF, Flow cyt
Goat anti-rabbit IgG (H+L), Cy3 conjugate SA00009-2 200ul* IF, Flow cyt
Rabbit anti-goat IgG (H+L), Cy3 conjugate SA00009-4 200ul* IF, Flow cyt

* In Europe, only 100ul vials are available.

Nano-Secondaries®

High resolution and low background

Nano-Secondaries® are the next level of secondary antibodies. They enable cleaner images with higher resolution. They consist of VHHs, also named Nanobodies, conjugated to Alexa Fluor® dyes. Nano-Secondaries have high affinities and bind to primary antibodies in a subclass and species-specific manner.

Product Name
Catalog No.
Size
Applications
Alexa Fluor® 488 Nano-secondary Antibody
      
Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 488 sms1AF488-1 10ul, 100ul IF, SRM, WB, ELISA
Alpaca anti-mouse IgG2b, Fc-specific recombinant VHH, Alexa Fluor® 488 sms2bAF488-1 10ul, 100ul IF, SRM, WB, ELISA
Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 srbAF488-1 10ul, 100ul IF, SRM, WB, ELISA
       
Alexa Fluor® 568 Nano-secondary Antibody
      
Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 568 sms1AF568-1 10ul, 100ul IF, SRM, WB, ELISA
Alpaca anti-mouse IgG2b, Fc-specific recombinant VHH, Alexa Fluor® 568 sms2bAF568-1 10ul, 100ul IF, SRM, WB, ELISA
Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 srbAF568-1 10ul, 100ul IF, SRM, WB, ELISA
       
Alexa Fluor® 647 Nano-secondary Antibody
      
Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 647 sms1AF647-1 10ul, 100ul IF, SRM, WB, ELISA
Alpaca anti-mouse IgG2b, Fc-specific recombinant VHH, Alexa Fluor® 647 sms2bAF647-1 10ul, 100ul IF, SRM, WB, ELISA
Alpaca anti-mouse IgG3, Fab-specific recombinant VHH, Alexa Fluor® 647 sms3AF647-1 10ul, 100ul IF, SRM, WB, ELISA
Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 srbAF647-1 10ul, 100ul IF, SRM, WB, ELISA

SRM = Super-resolution Microscopy

 

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