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ChromoTek Nanobody-based reagents

ChromoTek offers premium Nanobody-based research tools that provide a higher level of performance than conventional IgG antibodies. Alpaca Nanobodies are the smallest antibodies available (15kDa), have very high affinity, and are exceptionally stable.

ChromoTek's Nanobodies are optimized for:

Chromotek logo with alpaca

ChromoTek recently became part of Proteintech Group. All ChromoTek Nanobody-based reagents are now available on ptglab.com and chromotek.com. 

Nano-Traps

Affinity beads for immunoprecipitation (IP)

Nano-Traps are ideal for fast and efficient immunoprecipitations (IP). Nano-Traps consist of VHHs, also termed Nanobodies, coupled to beads.  

The immunoprecipitation (IP) validation of GFP with ChromoTek's GFP-Trap Agarose beads

Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound

Nano-Traps for IP:

  • No heavy & light antibody chains
  • Stringent washing conditions
  • Low host cell protein background
  • Very efficient pulldown, no protein in flow through (FT)
  • High affinity to bind even proteins expressed at low levels
  • Short incubation (30-60 min)
  • Recombinantly expressed and validated

 Applications:

  • Immunoprecipitation/ Co-IP
  • Mass spectrometry
  • On-bead enzyme assays
  • ChIP, RIP analysis


More details on Nano-Traps available on ChromoTek. 

Product Formats (Product citations in parentheses)
GFP-Trap® Agarose (2,515), Magnetic Agarose (593), Dynabeads (3), Multiwell Plate (14)
GST-Trap
 Agarose (9)
Halo-Trap
 Agarose
MBP-Trap
 Agarose (5)
Mdm4/HdmX-Trap
 Agarose
MK2-Trap Agarose
mNeonGreen-Trap Agarose (3), Magnetic Agarose
Myc-Trap® Agarose (60), Magnetic Agarose (22)
p53-N-term-Trap Agarose (5)
p53-C-term-Trap Agarose (1)
PARP1-Trap Agarose (5)
RFP-Trap® Agarose (148), Magnetic Agarose, Dynabeads
SNAP/CLIP-tag®-Trap Agarose (1)
Spot-Trap® Agarose (1), Magnetic Agarose, Dynabeads
TurboGFP-Trap Agarose (2), Magnetic Agarose
V5-Trap™ Agarose, Magnetic Agarose

Kits including lysis, wash, and elution buffers are available for all Nano-Trap products. 

 

Nano-Secondaries

High resolution and low background

Nano-Secondaries® are the next level of secondary antibodies. They enable cleaner images with higher resolution. They consist of VHHs, also named Nanobodies, conjugated to Alexa Fluor® dyes. Nano-Secondaries have high affinities and bind to primary antibodies in a subclass and species-specific manner.

Staining image of Tubulin-GFP immunostained with rabbit anti-GFP PABG1 antibody and ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor 647

Tubulin-GFP immunostained with rabbit anti-GFP PABG1 antibody and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor 647 [CTK0101, CTK0102]

A novel class of secondary antibodies

  • Faster staining: One-step incubation
  • Higher resolution
  • Low background
  • No cross-reactivity and high subclass specificity

 Applications

  • Immunofluorescence
  • Super-resolution microscopy
  • Western blotting
  • Flow cytometry

 

Get more details on Nano-Secondaries here on ChromoTek.

Product Formats (Product citations in parentheses)
Nano-Secondary® alpaca anti-human IgG, recombinant VHH [CTK0101, CTK0102] Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647
Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH [CTK0103, CTK0104] Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647 (1)
Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH [CTK0105, CTK0106] Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647
Nano-Secondary® alpaca anti-mouse IgG3, recombinant VHH [CTK0107] Alexa Fluor® 647
Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH [CTK0101, CTK0102] Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647

 

Chromobodies

Intracellular Nanobodies for live-cell analysis in real-time

Chromobodies are small fluorescent probes to visualize proteins in live-cells. Chromobodies are intracellular fluorescent alpaca Nanobodies fused to fluorescent proteins. Chromobody products are DNA plasmids for transfection.

A Chromobody is a genetic fusion of a target-specific VHH from alpaca and a fluorescent protein (TagRFP or TagGFP, EGFP) ChromoTek’s Chromobodies are derived from the antigen binding domain VHH, also called Nanobodies, of alpaca heavy chain antibodies. Chromobodies are genetic fusions of a VHH  and a fluorescent protein. The DNA plasmid coding for the Chromobody can be transfected into cells and the Chromobody is then intracellularly expressed by the transfected cell.

 

Dynamic live-cell analysis of endogenous proteins:

  • Fast no-wash assay
  • No overexpression of the protein of interest
  • No cytotoxicity and artificial effects

Applications:

  • Live-cell imaging of endogenous proteins
  • High content analysis of cells, tissues, whole organs (sections), and organisms (sections)
  • Investigation of small molecules in secondary screens – either as biomarkers or controls

 Get more information on Chromobodies from ChromoTek here.

Product Plasmids (Product citations in parentheses)
Actin-Chromobody® TagGFP2 (15), TagRFP (5)
Nuclear Actin-Chromobody® TagGFP2
Cell Cycle-Chromobody® TagRFP (11)
Dnmt1-Chromobody® TagGFP2, TagRFP (1)
Histone-Chromobody® eGFP (1)
Lamin-Chromobody® TagGFP2 (5)
PARP1-Chromobody® TagGFP2, TagRFP (2)
Vimentin-Chromobody® TagGFP (2)

Histone Chromobodies are commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment.

 

Nano-Boosters & Nano-Labels

Higher resolution and better tissue penetration

ChromoTek's Nano-Boosters & Nano-Labels are fluorescent probes. They consist of a VHH, also called a Nanobody, conjugated to a fluorescent dye such as Alexa Fluor 647. Nano-Boosters and Labels are ideal probes for super-resolution imaging.

Staining image of HeLa cells transfected with Tom70-eGFP and PCNA-mRFP and stained with GFP-Booster Alexa Fluor 488 (green) and RFP-Booster Alexa Fluor 568 (red) recorded at the Core Facility Bioimaging, LMU Munich

Benefits  

  • Higher labeling density
  • Better tissue penetration

 

Fig. HeLa cells transfected with Tom70-eGFP and PCNA-mRFP and stained with GFP-Booster Alexa Fluor 488 (green) and RFP-Booster Alexa Fluor 568 (red). Images were recorded at the Core Facility Bioimaging, LMU Munich. Scale bar 10 µm.

Nano-Booster & Nano-Label for higher image resolution:

  •   Superior accessibility and labeling of epitopes in crowded cellular/organelle environments
  •   Minimal linkage error: Less than 2 nm epitope-label displacement
  •   GFP- and RFP-Boosters stabilize, retain, and enhance signals of fluorescent proteins
  •   Recombinantly expressed and validated

Applications:

  • Immunofluorescence (IF)
  • Light-sheet microscopy imaging including cleared tissue, organ, and animal (vDISCO) staining
  • Super-resolution microscopy, confocal and epi-fluorescence microscopy 

 Find out more about Nano-Boosters and Nano-Labels on ChromoTek.

Product Formats (Product citations in parentheses)
GFP-Booster Alexa Fluor® 488 (12), Alexa Fluor® 568 (1), Alexa Fluor® 647 (9), ATTO488 (138), ATTO594 (15), ATTO647N (27)
Histone-Label ATTO488 (1)
RFP-Booster Alexa Fluor® 568, Alexa Fluor® 647 (1), ATTO594 (28), ATTO647N (7)
Spot-Label® ATTO488, ATTO594, Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647
Vimentin-Label ATTO488 (2)

Histone Label is commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment.

 

Nano-Caps

Protein purification resins

ChromoTek’s Nano-Caps® for protein purification: They consist of Nanobodies/ VHHs conjugated to agarose. Nano-Caps® are optimized for effective and cost-efficient purification.

Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in batch format. Elution with Spot-peptide at 4°C

Purification of Spot-tagged protein. Elution with Spot-peptide at 4°C

Benefits

  • One-step protein purification
  • Efficient elution
  • Frequent regeneration for multiple reuse
  • High binding capacity

Applications

  • Protein purification

 

Get more info on Nano-Caps® from ChromoTek here.

Products Format
Spot-Cap Agarose beads

Nano-CaptureLigands

Site-directed immobilization of antibodies

Nano-CaptureLigands are optimized to specifically immobilize human, mouse, and rabbit antibodies to streptavidin/avidin. Nano-CaptureLigands are biotinylated VHHs/ Nanobodies with high affinity.

ChromoTek Nano-CaptureLigand (in green) immobilizing an antibody (in orange) on a biosensor

Nano-CaptureLigand (green) immobilizing an antibody (orange) on a biosensor

Advanced immobilization of antibodies

  • Gentle and site-directed immobilization of antibodies
  • Works with crude samples
  • Multiple regeneration cycles
  • No antibody biotinylation required

Applications

Antibody capture for

  • BLI (biolayer interferometry)
  • SPR (surface plasmon resonance)
  • ELISA (enzyme-linked immunosorbent assays)

 

Find out more about Nano-CaptureLigands here at ChromoTek.com 

Product Formats
Nano-CaptureLigand human Ig, lambda Biotinylated
Nano-CaptureLigand human IgE Biotinylated
Nano-CaptureLigand human IgG Biotinylated
Nano-CaptureLigand mouse IgE Biotinylated
Nano-CaptureLigand mouse IgG1 Biotinylated
Nano-CaptureLigand mouse IgG2a Biotinylated
Nano-CaptureLigand mouse IgG2b Biotinylated
Nano-CaptureLigand rabbit IgG Biotinylated

Nanobodies/ VHHs

Unconjugated primary single-domain antibodies

VHHs, also termed Nanobodies, are derived from heavy chain only antibodies of Camelids. ChromoTek’s VHHs are well characterized and validated. Because the molecular weight of these single-domain antibodies (sdAb) is just about 15 kDa, VHHs are only one-tenth the size of a conventional IgG antibody (150 kDa) or less than a third the size of a Fab fragment (50 kDa).

GFP VHH binds to GFP

GFP VHH binds to GFP

Properties

  • Small size
  • Single poly-peptide chain structure

Application

Suitable for conjugation to dyes, biotin, beads, surfaces etc. via NHS ester reaction

 

More Nanobodies/ VHHs information here on ChromoTek.

Product Formats (Product citations in parentheses)
GFP VHH Unconjugated (23)
GFP VHH, biotinylated Biotinylated
GST VHH Unconjugated (9)
Halo VHH Unconjugated
Histone VHH Unconjugated
MBP VHH Unconjugated
MK2 VHH Unconjugated
mNeonGreen VHH Unconjugated
Myc VHH Unconjugated
p53 N-term VHH Unconjugated
p53 C-term VHH Unconjugated
PARP1 VHH Unconjugated
RFP VHH Unconjugated (4)
SNAP/CLIP-tag® VHH Unconjugated
Spot VHH Unconjugated (1)
TurboGFP VHH Unconjugated
V5 VHH Unconjugated
Vimentin VHH Unconjugated

 

Spot-Tag

Spot-Tag for immunoprecipitation, protein purification & immunofluorescence

The Spot-Tag® System is developed for multiple capture and detection applications. The Spot-Tag is a short peptide tag with 12 amino acids (PDRVRAVSHWSS). The Spot-Tag is bound by application-optimized reagents, which are based on Spot Nanobodies.

Spot-nanobody in green binding to spot peptide

Spot-Nanobody (green) binds to Spot Peptide

Immunoprecipitation (IP) / Co-IP: Spot-Trap

Co-IP/MS: Spot-Trap and iST Spot-Trap Kit

Protein purification: Spot-Cap

Immunofluorescence: Spot-Label

Western Blotting: Spot-Label or Spot VHH

 

For more details on Spot-Tag®, visit ChromoTek.

Product Formats (Product citations in parentheses)
Spot-Cap Agarose beads
Spot-Label® ATTO488, ATTO594, Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647
Spot-Trap® Agarose (1), Magnetic Agarose, Dynabeads
Spot VHH Unconjugated

 

For research use only.

ChromoTek’s products and technology are covered by granted patents and pending applications owned by or available to ChromoTek GmbH by exclusive license.


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