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ChromoTek offers premium Nanobody-based research tools that provide a higher level of performance than conventional IgG antibodies. Alpaca Nanobodies are the smallest antibodies available (15kDa), have very high affinity, and are exceptionally stable.
ChromoTek's Nanobodies are optimized for:
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ChromoTek recently became part of Proteintech Group. All ChromoTek Nanobody-based reagents are now available on ptglab.com and chromotek.com.
Nano-Traps are ideal for fast and efficient immunoprecipitations (IP). Nano-Traps consist of VHHs, also termed Nanobodies, coupled to beads.
Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound |
Nano-Traps for IP:
Applications:
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More details on Nano-Traps available on ChromoTek.
Kits including lysis, wash, and elution buffers are available for all Nano-Trap products.
Nano-Secondaries® are the next level of secondary antibodies. They enable cleaner images with higher resolution. They consist of VHHs, also named Nanobodies, conjugated to Alexa Fluor® dyes. Nano-Secondaries have high affinities and bind to primary antibodies in a subclass and species-specific manner.
Tubulin-GFP immunostained with rabbit anti-GFP PABG1 antibody and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor 647 [CTK0101, CTK0102] |
A novel class of secondary antibodies
Applications
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Get more details on Nano-Secondaries here on ChromoTek.
Product | Formats (Product citations in parentheses) |
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH [CTK0101, CTK0102] | Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647 |
Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH [CTK0103, CTK0104] | Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647 (1) |
Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH [CTK0105, CTK0106] | Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647 |
Nano-Secondary® alpaca anti-mouse IgG3, recombinant VHH [CTK0107] | Alexa Fluor® 647 |
Chromobodies are small fluorescent probes to visualize proteins in live-cells. Chromobodies are intracellular fluorescent alpaca Nanobodies fused to fluorescent proteins. Chromobody products are DNA plasmids for transfection.
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ChromoTek’s Chromobodies are derived from the antigen binding domain VHH, also called Nanobodies, of alpaca heavy chain antibodies. Chromobodies are genetic fusions of a VHH and a fluorescent protein. The DNA plasmid coding for the Chromobody can be transfected into cells and the Chromobody is then intracellularly expressed by the transfected cell. |
Dynamic live-cell analysis of endogenous proteins:
Applications:
Get more information on Chromobodies from ChromoTek here.
Product | Plasmids (Product citations in parentheses) |
Actin-Chromobody® | TagGFP2 (15), TagRFP (5) |
Nuclear Actin-Chromobody® | TagGFP2 |
Cell Cycle-Chromobody® | TagRFP (11) |
Dnmt1-Chromobody® | TagGFP2, TagRFP (1) |
Histone-Chromobody® | eGFP (1) |
Lamin-Chromobody® | TagGFP2 (5) |
PARP1-Chromobody® | TagGFP2, TagRFP (2) |
Vimentin-Chromobody® | TagGFP (2) |
Histone Chromobodies are commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment.
ChromoTek's Nano-Boosters & Nano-Labels are fluorescent probes. They consist of a VHH, also called a Nanobody, conjugated to a fluorescent dye such as Alexa Fluor 647. Nano-Boosters and Labels are ideal probes for super-resolution imaging.
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Benefits
Fig. HeLa cells transfected with Tom70-eGFP and PCNA-mRFP and stained with GFP-Booster Alexa Fluor 488 (green) and RFP-Booster Alexa Fluor 568 (red). Images were recorded at the Core Facility Bioimaging, LMU Munich. Scale bar 10 µm. |
Nano-Booster & Nano-Label for higher image resolution:
Applications:
Find out more about Nano-Boosters and Nano-Labels on ChromoTek.
Product | Formats (Product citations in parentheses) |
GFP-Booster | Alexa Fluor® 488 (12), Alexa Fluor® 568 (1), Alexa Fluor® 647 (9), ATTO488 (138), ATTO594 (15), ATTO647N (27) |
Histone-Label | ATTO488 (1) |
RFP-Booster | Alexa Fluor® 568, Alexa Fluor® 647 (1), ATTO594 (28), ATTO647N (7) |
Spot-Label® | ATTO488, ATTO594 |
Vimentin-Label | ATTO488 (2) |
Histone Label is commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment.
ChromoTek’s Nano-Caps® for protein purification: They consist of Nanobodies/ VHHs conjugated to agarose. Nano-Caps® are optimized for effective and cost-efficient purification.
Purification of Spot-tagged protein. Elution with Spot-peptide at 4°C |
Benefits
Applications
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Get more info on Nano-Caps® from ChromoTek here.
Products | Format |
Spot-Cap | Agarose beads |
Nano-CaptureLigands are optimized to specifically immobilize human, mouse, and rabbit antibodies to streptavidin/avidin. Nano-CaptureLigands are biotinylated VHHs/ Nanobodies with high affinity.
Nano-CaptureLigand (green) immobilizing an antibody (orange) on a biosensor |
Advanced immobilization of antibodies
Applications Antibody capture for
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Find out more about Nano-CaptureLigands here at ChromoTek.com
VHHs, also termed Nanobodies, are derived from heavy chain only antibodies of Camelids. ChromoTek’s VHHs are well characterized and validated. Because the molecular weight of these single-domain antibodies (sdAb) is just about 15 kDa, VHHs are only one-tenth the size of a conventional IgG antibody (150 kDa) or less than a third the size of a Fab fragment (50 kDa).
GFP VHH binds to GFP |
Properties
Application Suitable for conjugation to dyes, biotin, beads, surfaces etc. via NHS ester reaction |
More Nanobodies/ VHHs information here on ChromoTek.
The Spot-Tag® System is developed for multiple capture and detection applications. The Spot-Tag is a short peptide tag with 12 amino acids (PDRVRAVSHWSS). The Spot-Tag is bound by application-optimized reagents, which are based on Spot Nanobodies.
Spot-Nanobody (green) binds to Spot Peptide |
Immunoprecipitation (IP) / Co-IP: Spot-Trap Co-IP/MS: Spot-Trap and iST Spot-Trap Kit Protein purification: Spot-Cap Immunofluorescence: Spot-Label Western Blotting: Spot-Label or Spot VHH |
For more details on Spot-Tag®, visit ChromoTek.
Product | Formats (Product citations in parentheses) |
Spot-Cap | Agarose beads |
Spot-Label® | ATTO488, ATTO594 |
Spot-Trap® | Agarose (1), Magnetic Agarose, Dynabeads |
Spot VHH | Unconjugated |
For research use only.
ChromoTek’s products and technology are covered by granted patents and pending applications owned by or available to ChromoTek GmbH by exclusive license.