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SignalBright Chemiluminescent Substrate
Introduction

To detect antibody binding to a protein of interest during a western blot (WB) experiment, a signal generation system is required. The most common method of signal generation for WB is a primary or secondary antibody conjugated to the enzyme horseradish peroxidase (HRP), which emits light in the presence of a luminol and a peroxide buffer (Figure 1). This enzyme-substrate combination only produces light during the reaction, after which signal output diminishes.

Figure 1: Signal generation in chemiluminescent western blotting.

A secondary antibody with an HRP conjugate enables the detection of a target protein by oxidizing its substrate luminol with the emission of light as a by-product.

Proteintech is excited to launch our new range of highly sensitive chemiluminescent substrates.  Our SignalBirght Chemiluminescent Substrate range of SignalBright Pro (PK10011), SignalBright Plus (PK10012), and SignalBright Max (PK10013) is perfect for the detection of femtogram levels of protein. See the table of comparisons below to select which substrate is right for you.

Comparisons for Proteintech’s SignalBright substrates

Comparison table of Proteintech's SignalBright enhanced chemiluminescent substrate

Proteintech’s SignalBright Pro (PK10011), SignalBright Plus (PK10012), and SignalBright Max (PK10013) Enhanced Chemiluminescent Substrate have different levels of sensitivity and signal duration (see above table). In particular, our SignalBright has unparalleled performance to competitor products with highly sensitive detection, minimal optimization required, and long, stable signal duration.

 

Comparison of Proteintech's SignalBright Enhanced Chemiluminescent Substrate

Figure 2: Comparison of Proteintech’s SignalBright Pro (PK10011), SignalBright Plus (PK10012), and SignalBright Max (PK10013) Enhanced Chemiluminescent Substrate.

Serial dilutions of HeLa cell lysate. Primary antibody: Proteintech Beta Catenin 51067-2-AP, 1:20,000. Secondary: Quanta BioDesign HRP-Goat anti-Rabbit IgG (H&L) (11-0201-0503) at a 1:100,000 dilution. Exposure Time: 30 seconds.

 


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