4ul protein ladders were loaded in the gel with concentration 8%-18%. After Electrophoresis, the protein on the gel were transformed into the PVDF membrane. This image was taken by the iBright.

<strong>Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions</strong><br>The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE). The Image shows the migration pattern in the gel and after transfer to a PVDF membrane.

4ul protein ladders were loaded in the gel with concentration 8%-18%. After Electrophoresis, the protein on the gel were transformed into the PVDF membrane. This Image was taken by the iBright

Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in Figure 2.