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Proteintech provides a wide range of two-site sandwich ELISA kits as ready-to-use and highly sensitive immunoassay kits.

Here’s what you can expect from Proteintech ELISA kits:

  • Quick and simple to use.
  • High sensitivity and broad assay range
  • Detects quantitative protein level in serum, plasma or supernatant of cell lysates. 
  • Delivers consistent results with high reproducibility.
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AuthentiKine™  ELISA Kits

AuthentiKine™ ELISA kits are Proteintech’s premium line of ELISA kits for measuring minute quantities of human serum factors:

  • Ultra-sensitive kits between 10X and 1000X more sensitive than kits sold by leading competitors.

  • The only kits made using actual human proteins from state-of-the-art HumanKine® human cell-expressed cytokines and growth factors.

To achieve superior sensitivity for human samples, these kits are built from the ground up using    HumanKine® human-cell expressed cytokines and growth factors, in contrast to the industry standard of E. coli-derived proteins.

AuthentiKine™ ELISA kit development starts with production of HumanKine® immunogens, which are human proteins with native glycosylation and processing. Due to the highest quality immunogens in the industry, the capture and detection antibodies have the highest affinity for human proteins. To best mimic the human sample analyte for optimizing the antibody parameters, HumanKine® proteins are used as the standard. This means that the antibody manufacturing and conditions for optimizing are built around using human proteins as they are in nature, resulting in unparalleled sensitivity and accuracy.

Proteintech's TNF alpha AuthentiKine™ ELISA kit’s sensitivity vs leading competitor
Minimum Detectable Dose (pg/mL)
Target Fold better sensitivity Proteintech Leading Competitor
Human IFN-gamma 20X 0.4 8
Human Cystatin C 10X 10.5 102

Comparison of Proteintech’s AuthentiKine™ ELISA kits’ sensitivity versus top competitor’s kits determined by adding two standard deviations to the concentration corresponding to the mean optical density of 20 zero standard replicates.

AuthentiKine™ ELISA kit product range
ELISA Kit Catalog number Sample Type Sensitivity
Human IL-6   ELISA kit KE00139  serum, plasma, cell culture supernatant    1.2
Human IFN-gamma  ELISA kit KE00146 serum, plasma, cell culture supernatant  0.4
Human Cystatin C  ELISA kit KE00150 serum, plasma, cell culture supernatant, urine, saliva and human milk 10.5
Human TNF-alpha  ELISA kit KE00154 serum, plasma, cell culture supernatant  8.6
Human HGF  ELISA kit KE00168 serum, plasma, cell culture supernatant 260
Human IL-10  ELISA kit KE00170 serum, plasma, cell culture supernatant 2.3
Human beta-NGF  ELISA kit KE00174 Serum, plasma, Cell culture supernatants 0.1
Human IL-27 ELISA Kit KE00182 Serum, plasma 0.1
Human M-CSF ELISA Kit KE00184 Serum, Plasma, Cell culture supernatants, Urine, Saliva 1.1


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