Blog
How to write an industry resume/CV
Are you a life-scientist looking to change your career path? Learn how to write an industry resume/CV
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How to Identify Activated Microglia
Proteintech experts share ways to quantify your microglial cell population
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Building research careers in a post-COVID world
Creative strategies used by early-career researchers during the pandemic to establish themselves could launch careers after the pandemic fades.
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Primary Cilia and Regulation of Blood Pressure (“The Silent Killer”)
Discovering cilia’s role as a regulator of arterial blood vessels and its involvement in disease.
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Imaging the Rainbow: Multiplexing with Same-Species Antibodies for Immunofluorescence
Guest Blog Post by Derek Sung (IG: @Immunofluoresence)
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How to prepare for a PhD viva - Ten tips for success
Written by Tiago Marcos, PhD, University of Edinburgh.
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7 Tips to successfully culture primary rodent neurons
Proteintech experts provide seven tips for improving the integrity of your primary neuronal cultures.
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Overview of the vascular system and the importance of endothelial cells
Written by Kathryn Green, PhD student at The University of Nottingham.
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Proteintech interview with an early-career researcher: Odetta Antico
Odetta Anitco from the University of Dundee, Scotland, speaks to Proteintech about her scientific career so far, her recent paper in Science Advances, and how to handle setbacks in research.
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Chromatin Immunoprecipitation (ChIP) with RFP-tagged or mCherry-tagged proteins
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New anti-mNeonGreen antibody & Nano-Trap for Immunofluorescence & Immunoprecipitation
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Characterization of COVID-19 antibodies by Bio-Layer Interferometry using Nano-CaptureLigands
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Spot-Trap or Spot-Cap?
Our Sales Rep answers one of the most frequent customer questions
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Do you purify a special protein?
Why Spot-Tag can be used to purify any protein
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Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins
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New nuclear Actin-Chromobody® plasmid
It is our pleasure to announce the availability of a new member of our Actin Chromobody® plasmid family:
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How to overcome the limitations of antibody-based affinity resins for protein purification
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An alternative Nanobody format that combines its special epitope binding properties with the detection options for Fc-domains
A new Swiss army knife for immunofluorescence: Chimeric heavy chain antibodies
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GFP-Booster: GFP Nanobody for better images in immunofluorescence
Higher resolution and better tissue penetration
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How to immunoprecipitate Flag®-tagged proteins
Flag®-tag (or DYKDDDDK-tag) is a commonly used short peptide tag for multiple applications such as immunoprecipitation (IP), protein purification, immunofluorescence, and Western blotting (WB).
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Advantages and limitations of different antibody formats in immunoprecipitation
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Why is the Fab-Trap™ so Fab-ulous?
ChromoTek’s Fab-Trap™ is an IP reagent based on an antibody Fab-fragment.
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Antibody Specificity and affinity in Immunoprecipitation
What is antibody specificity and affinity? And why is it important for immunoprecipitation?
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Replication Race: Scientists unravel why Omicron spreads faster
Written by Tiago Marcos Ph.D, guest author from the University of Edinburgh.
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Co-immunoprecipitation troubleshooting, tips and tricks
Co-immunoprecipitation (Co-IP) describes the isolation of a protein and its binding partners from a cell extract using a Nanobody or antibody that is bound to beads. The protein, that directly interacts with the Nanobody, or antibody beads is called “bait”. The binding partner that is indirectly precipitated is called “prey”.
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How to conduct a Co-immunoprecipitation (Co-IP)
Co-IP describes the immunoprecipitation of a protein of interest and its interacting partners.
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Green fluorescent protein (GFP) in plant research
Why does GFP work so well in plants?
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TurboGFP: Properties, Sequence, MW, origin
Everything you need to know about TurboGFP: properties and applications
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Explore cytokeratin dynamics with CoraLite conjugates
Want to learn more about Cytokeratins and their role in disease development? Introducing CoraLite conjugated antibodies against cytokeratins.
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GFP (green fluorescent protein): Properties, origin, specifications, tips
Everything you need to know about GFP: properties and applications
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V5 Tag: Properties
V5 Tag: Properties, origin, specifications, antibodies & Nanobodies
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Halotag
HaloTag is a protein tag. The HaloTag protein can be fused to your protein of interest (POI) which enables it for cellular and biochemical analysis. It can also be used for live cell imaging.
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SNAP-tag and CLIP-tag overview
SNAP-tag and CLIP-tag are self-labeling protein tags. The SNAP-tag protein and the CLIP-tag protein can be fused to a protein of interest (POI) and used for cellular and biochemical analysis.
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mNeonGreen vs GFP
mNeonGreen: A green fluorescent protein that is not a GFP variant
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Detecting low abundance proteins via Western Blot
Detecting low abundance proteins can be a challenge - here's how to optimise your Western Blot.
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How Nanobodies could advance Sars-CoV diagnostics
Teresa Wagner and Ulrich Rothbauer discuss the development of Nanobodies against SARS-CoV-2 and why Nanobodies are versatile alternatives to classical antibodies in SARS-CoV-2 research.
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The highly adaptive cytoskeleton
The cytoskeleton is a highly important structure present in the cytoplasm of all cells. Learn how this is integral for many cellular functions, and the many ways researchers can study the cytoskeleton in our blog.
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Mouse-on-mouse for Immunohistochemistry: tips and tricks
Performing IHC using a mouse derived antibody on mouse tissue can be daunting due to the potential for off-target staining. Learn how to mitigate these issues and perform mouse-on-mouse staining with confidence.
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Want to upgrade your immunofluorescence workflow? Go Direct!
Direct vs Indirect Immunofluorescence
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New ways to study the elusive exosome
Written by Poppy Nathan, Proteintech Field Marketing Representative and PhD Student at the University of Birmingham
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Ask a Flow Cytometry Expert: Your Questions Answered
Dr. Gareth Howell, Senior Experimental Officer and Flow Core Facility Manager at the University of Manchester answers your questions.
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Navigating a PhD in a Pandemic
Guest blogger Francesca Lewns shares her tips and advice for navigating a PhD during a global pandemic
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To dye or not to dye? Understanding the different viability dyes available for flow cytometry
Dead cell exclusion in flow cytometry is crucial for reliable and accurate results. This blog will help you understand which reagents are best for your experiment, providing a comprehensive overview of the popular products available to help you find "another way to dye".
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Seizing the day: How to save time on your immunoassay experiments
When time in the lab is limited, learn how to save precious hours on your IP, IF, and western blotting experiments.
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Neuropilin-1 joins ACE2 as SARS-CoV-2 gateway
Using Proteintech and Chromotek antibodies, Davy et al published a landmark paper in Science detailing how Neuropilin-1 is required for SARS-CoV-2 host cell entry.
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Intracellular Flow Cytometry Staining Protocol
Understand the different approaches to intracellular staining for flow cytometry in this "do-it-yourself" guide to fix and perm.
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Interview with an early-career scientist: Dr Chaitali Mukherjee
Hear from an early-career scientist about her first-author publication in Cell Metabolism and her academic journey
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Exosomes: A big role for a small player
Exosomes uncovered. Recent research and the role of exosomes in disease
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What do you get when you buy a GMP-grade product?
GMP Manufacturing, benefits, and ISO certifications explained
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Comparing BCA, Bradford, and UV protein quantification methods for scientists
Understanding the pros and cons of BCA, Bradford, and UV methods
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Interview with an early-career scientist: Dr Nadav Weinstock
Hear from the lead author of a landmark paper in Neuron about his research into Krabbe Disease
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How to reduce autofluorescence
Understand the causes behind autofluorescence and how to deal with this problem.
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The advantages of CoraLite direct staining for IF
This seminar was originally recorded on May 11, 2020 as part of the MetroFlow group’s corporate member seminar series. Dr. William Olds, Scientific Officer at Proteintech, highlights how CoraLite® antibodies can streamline your multiplex immunofluorescence staining.
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Serious about Serology: Understanding COVID19 Antibody Tests
Serious about serology: Understanding COVID19 Antibody Tests
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Cellular senescence and markers
How do cells come to be under "cellular arrest". Learn what is cell senescence and how it is relevant in disease.
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Neutralizing antibodies and their role in COVID-19 research
Weathering the Cytokine Storm
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How fellowships can advance your scientific career
Thoughts and advice to anyone thinking of applying for fellowships worldwide
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How to solve the genome DNA damage
DNA damage repair mechanisms: in the prevention of age-related diseases and tumorigenesis
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Dying to see what’s inside: Staining organelles
Visualizing the interior of organelles helps us to understand their molecular mechanisms and study cell abnormalities.
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Proteases for Western Blotting: Choosing the right tools for the job
How proteases can help you to better understand your Western blot results
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Tips and tricks for successful MASS spec experiments
Troubleshooting guide to address the most common obstacles during MASS spec experiments.
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What is the difference between necrosis and apoptosis?
Cell death is a naturally occurring phenomenon in multicellular organisms. Cells die as a result of internal and external stimuli.
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Life Scientist Appreciation Week University of Chicago
June 17-21 2019. #CelebrateLifeScientists #LSAW
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Missing pieces: DNA damage
Various environmental and endogenous factors cause DNA damage, resulting in cell degeneration, aging, or tumorigenesis.
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IF imaging: Widefield versus confocal microscopy
How to answer the experimental question in the most efficient manner.
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MASS spec essentials
Mass spectrometry (mass spec; MS) is a powerful method for analyzing macromolecules.
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The state of the international scientist in the U.S.
Supporting foreign-born scientists contributing to biomedical research in the U.S.
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Why studying abroad makes sense: Scientists share their story
Perspectives on Emigrating for Academic Career Advancement
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CRISPR-Cas9, TALENs and ZFNs - the battle in gene editing
Technological advances are crucial for innovative biological research. ZFNs, TALENs, and CRISPR/CRISPR-associated Cas have revolutionized genome editing.
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QUANTITATIVE MIRNA PROFILING: HOW TO CHOOSE THE BEST RNA SEQUENCING METHOD?
RNA sequencing (RNA-seq) is a widely used method employing next generation sequencing (NGS) to discover and quantify the abundance of RNA molecules in biological samples.
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Are Mesenchymal Stem Cells (MSCs) true stem cells?
MSCs have great potential and clinical significance for tissue engineering. They are adult multipotent cells capable of differentiating to different types of tissues.
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7 secrets to a successful scientific presentation
Fight the fear of presenting your scientific research.
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A closer look at Embryonic Stem Cells (ESCs) – biological concepts
Embryonic stem cells (ESCs) are derived from pre-implantation-stage embryos, usually from the inner cell mass of blastocyst-stage embryos.
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Why Human Cell Expressed Proteins Are Superior for Clinical Applications
For Humans...By Human Cells
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Cell fate commitment and the Waddington landscape model
Waddington’s epigenetic landscape has been used for decades to illustrate the progressive restriction of cell differentiation during development.
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How do I know if the antibody will cross-react?
Cross-reactivity occurs when an antibody raised against one specific antigen recognizes two antigens that have similar structural regions. In this blog, we draw on our experience as antibody experts to explain why this might happen, and provide you with the tools to determine cross-reactivity in your own experiments.
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5 Tips for immunostaining using multiple labels
A multiple immunostaining technique is a combination of individual antigen detections in order to study the expression and co-localization of two or more different antigens in the same sample.
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How does blood coagulate?
Blood is an essential fluid that carries out the critical functions of transporting oxygen and nutrients to our cells.
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ICF syndrome: A gene-silencing chromatin disorder
Immunodeficiency, Centromere region instability, Facial anomalies syndrome (ICF) is a rare autosomal recessive disease that damages the immune system.
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Induced pluripotent stem cells (iPSCs) and modeling neurodegenerative disease in the dish
iPSCs and their potential for resetting the biological clock.
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Polyclonal vs. monoclonal antibodies
Both types have their unique advantages and disadvantages and can be used in a wide variety of applications.
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Epigenetic implications in brain functions - Neuroepigenetics
Environmental factors, stress, learning or drug exposure lead to active regulation of DNA structure in the nervous system.
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Immunotherapy: boosting the immune system to fight rare cancers
Immunotherapy, how does it work and what do we know so far?
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Rett syndrome - the second leading cause of mental retardation in women
In 1966, Rett syndrome was for the first time described as a clinical issue.
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Protein or peptide antigen? Advantages and disadvantages
Peptide versus Protein Immunogens
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Proteintech supports young scientist to attend EMBL conference
Proteintech supports young scientists – Non-Coding Genome EMBL conference Heidelberg, Germany, 13–16 Sep 2017
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Top Tips for a Successful Transition to a Postdoc Role
Advice and tips given by postdocs from all over the world.
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How to Write a Good Scientific Abstract
An abstract is a written statement describing key facts about your research project.
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How to be a great lab manager: 6 tips
Our advice to help you be a great lab manager and manage your time better.
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WNT Pathway - Defining the Canon
How did scientists figure out such a complicated pathway?
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The Complexity of Proteins
How do proteins have so much functional diversity based upon only 20 building blocks?
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Secondary Antibody Selection
How to choose the best secondary antibody for your application
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Alzheimer's Disease Signaling Pathway
Download or request a pathway poster for your lab
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MAPK Pathway: New Poster Available
Download or request a pathway poster for your lab
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Post Translational Modifications: An Overview
Nature’s escape from genetic imprisonment
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Caspase Cascade Pathway: New Poster Available
Download a pathway poster for your lab
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The Incubator Podcast Highlights
Season 2 is here! Here's a re-cap of the topics discussed in Season 1
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Cancer stem cells as a key to cure cancer
CSCs are cancerous yet have properties of stem cells: self-renewal and the ability to differentiate into multiple cell types that occurs in tumors.
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How To Optimize Your ELISA Experiments
Technical tips for better ELISA results
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GSK3 pathway poster
Request a printed copy of the GSK signaling pathway for your lab.
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Autophagy in the Pathogenesis of Disease
Aging, cancer, inflammation, infection, the microenvironment, and neurodegenerative disease
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9 Tips to optimize your IF experiments
Want to improve your IF experiments? Our 9 tech tips will help you achieve successful Immunofluorescence staining
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Special offer for newsletter sign up
Sign up to the Proteintech regular eNewsletter to receive a free lab survival kit.
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7 Tech Tips For Successful ChIP Experiments
New to ChIP or just want to improve? Our tech tips will help you achieve successful ChIP experiments
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Proteintech Product Reviews
Share your feedback with the research community and receive an Amazon gift card or discount on your next order.
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Lysate Preparation: How Do I Optimize My Extraction?
Technical tips to improve your sample preparation for Western blot
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Antibody Comparison Sites: How to Find Your Perfect Antibody Faster
Find Your Perfect Antibody Faster
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Antibodies for Cilia Development
This article reviews a selection of proteins involved in cilia development, or ciliogenesis...
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A beginners guide to Immunohistochemistry
10 steps to get you started with immunohistochemistry
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Loading Control Antibodies for Western Blotting
Technical tips and related products
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Immunofluorescence: Tips for Immunostaining Cultured Cells
Expert advice on immunostaining
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8 Top Tips for Subcellular Fractionation
8 technical tips for successful subcellular fractionation
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Mitochondria: More than just the powerhouse of the cell
Introduction to mitochondria
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Career Development Podcast: The Incubator
Fret over your scientific career no longer. Proteintech is sponsoring a monthly career development podcast, The Incubator.
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I Just Want Your Extra Time And Your...KISS-1
Anti-KISS-1 rabbit polyclonal antibody
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Seeing Clearly: Tips For Staining Cleared Tissue With Antibodies
Tips For Staining Cleared Tissue With Antibodies
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7 Tips For Optimizing Your Western Blotting Experiments
How to optimize your western blot protocol
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8 Top Tips For Immunoprecipitation
8 tips on how to perform better IP experiments.
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Mitochondria: Center of Cellular Signaling and Energetic Balance
Mitochondrial markers
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Recalling when and where: How the brain processes episodic memory
Introduction
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10 tips on how to best optimize siRNA transfection
siRNA transfection is a powerful tool used to understand the mechanisms of gene regulation and molecular pathways.
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Fast Phospho-rward: Making Phosphoprotein Antibodies
Phosphorylation is a key way that cells toggle activity of their equipment, playing integral roles in processes spanning from embryonic development to apoptosis.
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Molecular markers for liver cancer
The Proteintech blog explores molecular markers for Hepatocellular carcinoma
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Can man's best friend help cure cancer
Humans are not the only species on earth that develop cancer; our beloved pets can also succumb to the ‘Big C’ too.
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Can a Bacterial 'Brain' Control a Robot?
More and more, we are developing technology to precisely control biological systems....
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Making the connection between brain and body
We’re connected, from head to toe...
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Tech Tips | In search of low molecular weight proteins
Western Blot tips for low molecular weight proteins
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