Career development
Example PhD interview questions and answers
How to succeed in your PhD interview with answers to frequently asked questions
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Go with the Flow - Make the most of this powerful technique
Discover all the ways flow cytometry can be used to take your research further
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Scientists Discover Dynein-Driven Motility in Primary Cilia!
Previously thought to be stationary, researchers from Washington University in St. Louis have identified that the movement of primary cilia on pancreatic cells assists in insulin secretion.
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How to succeed in an interview – Academia vs. Industry
Find out how to best prepare for an interview with top tips from our experts with successful academic and industry careers.
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Second Cancer After Breast Cancer
Explore why breast cancer survivors are more likely to develop a second cancer after the first one has been cured.
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Ask the Experts: Transitioning from Academia to Industry
Read some of the engaging Q&A session from our recent careers workshop on moving from an academic to industry position.
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Breast Cancer Awareness Month
October is an annual health campaign to promote early detection, reduce risk factors and support patients
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How to start your own business after a PhD: an insider’s perspective
Looking to move from academia to industry? Get advice from Dr. Chris Bullock, CEO and Co-Founder of QV Bioelectronics, on how to become an entrepreneur after finishing your PhD.
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How to write an industry resume/CV
Are you a life-scientist looking to change your career path? Learn how to write an industry resume/CV
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How to Identify Activated Microglia
Proteintech experts share ways to quantify your microglial cell population
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Building research careers in a post-COVID world
Creative strategies used by early-career researchers during the pandemic to establish themselves could launch careers after the pandemic fades.
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Primary Cilia and Regulation of Blood Pressure (“The Silent Killer”)
Discovering cilia’s role as a regulator of arterial blood vessels and its involvement in disease.
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Imaging the Rainbow: Multiplexing with Same-Species Antibodies for Immunofluorescence
Improve your IF multiplexing with these expert tips from our guest blogger, Derek Sung (IG: @Immunofluoresence)
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How to prepare for a PhD viva - Ten tips for success
Written by Tiago Marcos, PhD, University of Edinburgh.
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7 Tips to successfully culture primary rodent neurons
Proteintech experts provide seven tips for improving the integrity of your primary neuronal cultures.
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Overview of the vascular system and the importance of endothelial cells
Written by Kathryn Green, PhD student at The University of Nottingham.
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Important facts to know when using Nano-Secondary reagents
What I should know when I'm working with Secondary antibodies or Nano-Secondary reagents
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The importance of blocking when using Nano-Secondary reagents for IF
Blocking improves the sensitivity by reducing nonspecific background and therefore increases microscopy image quality. Insufficient blocking results in higher background noise.
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Virus research using GFP
References from the last 4 years in virus research using GFP and ChromoTek's GFP-Trap
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How to elute GFP-fusion protein from the GFP-Trap®
Tips and tricks
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The best anti-GFP antibody for immunoprecipitation: GFP-Trap
Comparison of GFP-Trap and anti-GFP IgG antibody
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Proteintech interview with an early-career researcher: Odetta Antico
Odetta Anitco from the University of Dundee, Scotland, speaks to Proteintech about her scientific career so far, her recent paper in Science Advances, and how to handle setbacks in research.
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Visualize the onset of metastasis with Chromobodies
The potential of the chromobody technology to study EMT in cell-based screening systems is reviewed by Maier et al., Scientific Reports 2015.
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Spot Capture and Detection Peptide Tag and Nanobody
The Spot Nanobody for multiple capture & detection applications of Spot-tagged proteins
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Actin Chromobody: Visualization of actin dynamics – NOT alteration
The ChromoTek Actin Chromobody is the only probe that does not interfere with actin filament dynamics.
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Chromatin Immunoprecipitation (ChIP) with RFP-tagged or mCherry-tagged proteins
RFP-Trap for ChIP analysis in order to detect enrichment of mCherry-tagged proteins at DNA binding sites
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New anti-mNeonGreen antibody & Nano-Trap for Immunofluorescence & Immunoprecipitation
Novel tools for the analysis of mNeonGreen fusion proteins
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Characterization of COVID-19 antibodies by Bio-Layer Interferometry using Nano-CaptureLigands
Binding of the immobilized COVID-19 antibodies to their target, the receptor-binding domain (RBD) of the SARS-CoV2 Spike protein, is characterized with regards to ka, kd, and KD.
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Nano-Booster FAQs
Frequently asked technical questions on GFP-and RFP-Booster
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Spot-Trap or Spot-Cap?
Our Sales Rep answers one of the most frequent customer questions
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Do you purify a special protein?
Why Spot-Tag can be used to purify any protein
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Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins
Publications using ChromoTek's GST-Trap in various applications
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New nuclear Actin-Chromobody® plasmid
It is our pleasure to announce the availability of a new member of our Actin Chromobody® plasmid family:
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Split fluorescent protein technology
The ChromoTek GFP-Trap has been successfully applied to multiple assays using different split GFP variants
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How to overcome the limitations of antibody-based affinity resins for protein purification
Spot-Cap for highly effective one-step protein purification
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What is one-step immunostaining?
This method reduces incubation time, hands-on time and the number of washing steps.
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Which beads should I use for my immunoprecipitation?
In detail, all beads have very low background binding due to the highly specific binding of the Nano-Traps.
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Tips and tricks for immunoprecipitation of low abundant proteins
For a successful IP, the abundance or expression level of the POI is crucial. If a POI is strongly expressed and highly abundant, then the IP will most likely be straightforward, with no difficulties.
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GFP and RFP-Booster for better immunofluorescence imaging
ChromoTek's GFP Nanobodies and RFP Nanobodies conjugated to fluorescent dyes
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Immunoprecipitation of Myc-tagged proteins – How it works
ChromoTek Myc-Trap® is an optimized tool for immunoprecipitation (IP/Co-IP)
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How fluorescent proteins can be applied in SARS-CoV-2 research
GFP or RFP and their derivatives are commonly used for virus research
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An alternative Nanobody format that combines its special epitope binding properties with the detection options for Fc-domains
A new Swiss army knife for immunofluorescence: Chimeric heavy chain antibodies
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GFP-Booster: GFP Nanobody for better images in immunofluorescence
Higher resolution and better tissue penetration
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How to immunoprecipitate Flag®-tagged proteins
Flag®-tag (or DYKDDDDK-tag) is a commonly used short peptide tag for multiple applications such as immunoprecipitation (IP), protein purification, immunofluorescence, and Western blotting (WB).
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Advantages and limitations of different antibody formats in immunoprecipitation
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Why is the Fab-Trap™ so Fab-ulous?
ChromoTek’s Fab-Trap™ is an IP reagent based on an antibody Fab-fragment.
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The most frequently cited monoclonal anti-RFP antibody
Immunofluorescence and immunohistochemistry with the ChromoTek's anti-RFP antibody [5F8]
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Immunoprecipitation without additional bands
Pull-down of proteins with Nano-Traps
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Conjugation of fluorescent dyes to Nanobodies
The conjugation of the binding domains of camelid heavy chain antibodies, also known as VHHs or Nanobodies, to fluorescent dyes is generating versatile tools for fluorescence microscopy applications like immunofluorescence (IF) and super resolution microscopy (SRM):
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Antibody Specificity and affinity in Immunoprecipitation
What is antibody specificity and affinity? And why is it important for immunoprecipitation?
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Replication Race: Scientists unravel why Omicron spreads faster
Written by Tiago Marcos Ph.D, guest author from the University of Edinburgh.
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Co-immunoprecipitation troubleshooting, tips and tricks
Co-immunoprecipitation (Co-IP) describes the isolation of a protein and its binding partners from a cell extract using a Nanobody or antibody that is bound to beads. The protein, that directly interacts with the Nanobody, or antibody beads is called “bait”. The binding partner that is indirectly precipitated is called “prey”.
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How to conduct a Co-immunoprecipitation (Co-IP)
Co-IP describes the immunoprecipitation of a protein of interest and its interacting partners.
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Green fluorescent protein (GFP) in plant research
Why does GFP work so well in plants?
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TurboGFP: Properties, Sequence, MW, origin
Everything you need to know about TurboGFP: properties and applications
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Fluorescent protein tags
Types of fluorescent protein tags and comparisons
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Explore cytokeratin dynamics with CoraLite conjugates
Want to learn more about Cytokeratins and their role in disease development? Introducing CoraLite conjugated antibodies against cytokeratins.
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GFP (green fluorescent protein): Properties, origin, specifications, tips
Everything you need to know about GFP: properties and applications
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Tags for protein purification
Overview over commonly used affinity tags in protein purification
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V5-Tag: Properties
V5-Tag: Properties, origin, specifications, antibodies & Nanobodies
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Flag-tag and 3x Flag-tag
Overview about specifications, properties, origin, sequence, size, specifications, etc. of Flag-tag
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Halotag
HaloTag is a protein tag. The HaloTag protein can be fused to your protein of interest (POI) which enables it for cellular and biochemical analysis. It can also be used for live cell imaging.
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SNAP-tag and CLIP-tag overview
SNAP-tag and CLIP-tag are self-labeling protein tags. The SNAP-tag protein and the CLIP-tag protein can be fused to a protein of interest (POI) and used for cellular and biochemical analysis.
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mNeonGreen vs GFP
mNeonGreen: A green fluorescent protein that is not a GFP variant
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Detecting low abundance proteins via Western Blot
Detecting low abundance proteins can be a challenge - here's how to optimize your Western Blot.
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How Nanobodies could advance Sars-CoV diagnostics
Teresa Wagner and Ulrich Rothbauer discuss the development of Nanobodies against SARS-CoV-2 and why Nanobodies are versatile alternatives to classical antibodies in SARS-CoV-2 research.
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The highly adaptive cytoskeleton
The cytoskeleton is a highly important structure present in the cytoplasm of all cells. Learn how this is integral for many cellular functions, and the many ways researchers can study the cytoskeleton in our blog.
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Mouse-on-mouse for Immunohistochemistry: tips and tricks
Learn how to mitigate issues with high background staining and perform mouse-on-mouse staining with confidence.
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Want to upgrade your immunofluorescence workflow? Go Direct!
Learn about the advantages of using directly conjugated primary antibodies for IF
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How to choose your recombinant proteins, cytokines & growth-factors
Considerations when choosing recombinant proteins for your research applications.
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New ways to study the elusive exosome
Written by Poppy Nathan, Proteintech Field Marketing Representative and PhD Student at the University of Birmingham
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Ask a Flow Cytometry Expert: Your Questions Answered
Dr. Gareth Howell, Senior Experimental Officer and Flow Core Facility Manager at the University of Manchester answers your questions.
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Navigating a PhD in a Pandemic
Tips and advice for navigating a PhD during a global pandemic, written by guest blogger Francesca Lewns.
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To dye or not to dye? Understanding the different viability dyes available for flow cytometry
Learn about the types of viability dyes available to help you achieve reliable and accurate results in flow cytometry.
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Seizing the day: How to save time on your immunoassay experiments
When time in the lab is limited, learn how to save precious hours on your IP, IF, and western blotting experiments.
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Neuropilin-1 joins ACE2 as SARS-CoV-2 gateway
Landmark paper in Science detailing how Neuropilin-1 is required for SARS-CoV-2 host cell entry, using Proteintech and Chromotek antibodies.
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Intracellular Flow Cytometry Staining Protocol
Understand the different approaches to intracellular staining for flow cytometry in this "do-it-yourself" guide to fix and perm.
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Interview with an early-career scientist: Dr Chaitali Mukherjee
Hear from an early-career scientist about her first-author publication in Cell Metabolism and her academic journey
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Using Flow Cytometry with Epigenetics
Experimental considerations for using flow cytometry to investigate epigenetics.
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Exosomes: A big role for a small player
Exosomes uncovered. Recent research and the role of exosomes in disease
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What do you get when you buy a GMP-grade product?
GMP Manufacturing, benefits, and ISO certifications explained
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Comparing BCA, Bradford, and UV protein quantification methods for scientists
Understanding the pros and cons of BCA, Bradford, and UV methods of protein quantification
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Interview with an early-career scientist: Dr Nadav Weinstock
Hear from the lead author of a landmark paper in Neuron about his research into Krabbe Disease
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How to reduce autofluorescence
Understand the causes behind autofluorescence and how to deal with this problem.
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The advantages of CoraLite direct staining for IF
Video highlights of how CoraLite® antibodies can streamline your multiplex immunofluorescence staining.
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Serious about Serology: Understanding COVID19 Antibody Tests
Serious about serology: Understanding COVID19 Antibody Tests
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Cellular senescence and markers
How do cells come to be under "cellular arrest"? Learn what cell senescence is and how it is relevant in disease.
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Neutralizing antibodies and their role in COVID-19 research
Weathering the Cytokine Storm
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How fellowships can advance your scientific career
Thoughts and advice to anyone thinking of applying for fellowships worldwide
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How to solve the genome DNA damage
DNA damage repair mechanisms: in the prevention of age-related diseases and tumorigenesis
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13 tips how to be more eco-friendly in the lab
Eco-friendly lab - Why Bother?
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Dying to see what’s inside: Staining organelles
Visualizing the interior of organelles helps us to understand their molecular mechanisms and study cell abnormalities.
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Proteases for Western Blotting: Choosing the right tools for the job
How proteases can help you to better understand your Western blot results
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Tips and tricks for successful Mass spec experiments
Troubleshooting guide to address the most common obstacles during mass spectrometry experiments.
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What is the difference between necrosis and apoptosis?
Understand the differences between regulated and unregulated cell death.
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Life Scientist Appreciation Week University of Chicago
June 17-21 2019. #CelebrateLifeScientists #LSAW
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Missing pieces: DNA damage
Various environmental and endogenous factors cause DNA damage, resulting in cell degeneration, aging, or tumorigenesis.
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IF imaging: Widefield versus confocal microscopy
An overview of microscopy techniques and advice on when to use each method.
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Mass spec essentials
An overview of mass spec sample preparation, sample purification and data analysis.
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The state of the international scientist in the U.S.
Supporting foreign-born scientists contributing to biomedical research in the U.S.
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Why studying abroad makes sense: Scientists share their story
Perspectives on Emigrating for Academic Career Advancement
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CRISPR-Cas9, TALENs and ZFNs - the battle in gene editing
Technological advances are crucial for innovative biological research. ZFNs, TALENs, and CRISPR/CRISPR-associated Cas have revolutionized genome editing.
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Quantitative MiRNA Profiling: How To Choose The Best RNA Sequencing Method?
RNA sequencing (RNA-seq) is a widely used method employing next generation sequencing (NGS) to discover and quantify the abundance of RNA molecules in biological samples.
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Are Mesenchymal Stem Cells (MSCs) true stem cells?
A guide to MSC characteristics, differentiation pathways and supporting reagents for MSC culture.
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7 secrets to a successful scientific presentation
Fight the fear of presenting your scientific research.
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A closer look at Embryonic Stem Cells (ESCs) – biological concepts
An overview of the history, features and applications of ESCs.
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Why Human Cell Expressed Proteins Are Superior for Clinical Applications
Learn about the advantages of using human cell expressed recombinant proteins in your clinical applications.
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Cell fate commitment and the Waddington landscape model
Waddington’s epigenetic landscape has been used for decades to illustrate the progressive restriction of cell differentiation during development.
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How do I know if the antibody will cross-react?
Cross-reactivity occurs when an antibody raised against one specific antigen recognizes two antigens that have similar structural regions. In this blog, we draw on our experience as antibody experts to explain why this might happen, and provide you with the tools to determine cross-reactivity in your own experiments.
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5 Tips for immunostaining using multiple labels
Our advice on microscope set-up, fluorophore choice and controls to improve your multi-label IF.
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How does blood coagulate?
Blood is an essential fluid that carries out the critical functions of transporting oxygen and nutrients to our cells.
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ICF syndrome: A gene-silencing chromatin disorder
Immunodeficiency, Centromere region instability, Facial anomalies syndrome (ICF) is a rare autosomal recessive disease that damages the immune system.
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Induced pluripotent stem cells (iPSCs) and modeling neurodegenerative disease in the dish
iPSCs and their potential for resetting the biological clock.
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Polyclonal vs. monoclonal antibodies
Both types have their unique advantages and disadvantages and can be used in a wide variety of applications.
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Epigenetic implications in brain functions - Neuroepigenetics
Environmental factors, stress, learning or drug exposure lead to active regulation of DNA structure in the nervous system.
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Immunotherapy: boosting the immune system to fight rare cancers
Immunotherapy: how does it work and what do we know so far?
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Rett syndrome - the second leading cause of mental retardation in women
An overview of Rett syndrome: clinical features, neuropathology and animal models.
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Protein or peptide antigen? Advantages and disadvantages
Peptide versus whole protein immunogens for antibody production
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Proteintech supports young scientist to attend EMBL conference
Proteintech supports young scientists – Non-Coding Genome EMBL conference Heidelberg, Germany, 13–16 Sep 2017
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Top Tips for a Successful Transition to a Postdoc Role
Advice and tips given by postdocs from all over the world.
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How to Write a Good Scientific Abstract
Our top tips and how to write an effective scientific abstract.
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How to be a great lab manager: 6 tips
Our advice to help you be a great lab manager and manage your time better.
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Type 2 Diabetes Pathogenesis
Does Insulin Resistance Cause hyperinsulinemia?
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WNT Pathway - Defining the Canon
How did scientists figure out such a complicated pathway?
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The Complexity of Proteins
How do proteins have so much functional diversity based upon only 20 building blocks?
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Secondary Antibody Selection
How to choose the best secondary antibody for your application
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Metabolic Regulation of Cell Death
A matter of life and (cell) death...
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Epigenetics Modifications - DNA methylation
DNA Methylation: An Overview
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Alzheimer's Disease Signaling Pathway
Download or request a pathway poster for your lab
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Epigenetics - DNA layers and chromatin modeling
Introduction to Epigenetics
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MAPK Pathway: New Poster Available
Download or request a pathway poster for your lab
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Post Translational Modifications: An Overview
Nature’s escape from genetic imprisonment
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Pathway Poster Library
Download or request a printed poster to decorate your lab
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Caspase Cascade Pathway: New Poster Available
Download a pathway poster for your lab
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The Incubator Podcast Highlights
Season 2 is here! Here's a re-cap of the topics discussed in Season 1
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Cancer stem cells as a key to cure cancer
A guide to cancer stem cells and the markers that can be used to isolate cell populations.
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How To Optimize Your ELISA Experiments
Technical tips for better ELISA results
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JAK/STAT Signaling: New Pathway Poster
Download a pathway poster for your lab
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GSK3 pathway poster
Request a printed copy of the GSK signaling pathway for your lab.
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Autophagy in the Pathogenesis of Disease
Aging, cancer, inflammation, infection, the microenvironment, and neurodegenerative disease
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9 Tips to optimize your IF experiments
Want to improve your IF experiments? Our 9 tech tips will help you achieve successful Immunofluorescence staining
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Special offer for newsletter sign up
Sign up to the Proteintech regular eNewsletter to receive a free lab survival kit.
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7 Tech Tips For Successful ChIP Experiments
New to ChIP or just want to improve? Our tech tips will help you achieve successful ChIP experiments
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Atrial and ventricular myocytes
A guide to atrial and ventricular myocyte markers
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Proteintech Product Reviews
Share your feedback with the research community and receive an Amazon gift card or discount on your next order.
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Lysate Preparation: How Do I Optimize My Extraction?
Technical tips to improve your sample preparation for Western blot
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Lysate Preparation: Why is RIPA Buffer Best for Western Blot
What is in a lysis buffer? Learn the purpose of each component.
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The Neurochemistry of Parkinson's Disease
An overview of brain neurochemistry in Parkinson's Disease, written by guest blogger Ashley Juavinett.
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Antibody Comparison Sites: How to Find Your Perfect Antibody Faster
A guide to finding the best antibody for your application
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Antibodies for Cilia Development
A guide to key targets in cilia development and ciliogenesis.
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MHC Class I: The Cancer Connection
The relationship between the major histocompatibility complex (MHC) and cancer.
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A beginners guide to Immunohistochemistry
12 steps to get you started with immunohistochemistry
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Loading Control Antibodies for Western Blotting
Discover the most appropriate loading controls for your Western blot experiments.
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Immunofluorescence: Tips for Immunostaining Cultured Cells
Improve your IF with these expert tips from Proteintech scientists.
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8 Top Tips for Subcellular Fractionation
8 technical tips for successful subcellular fractionation
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Mitochondria: More than just the powerhouse of the cell
An introduction to mitochondria in health and disease.
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Career Development Podcast: The Incubator
Fret over your scientific career no longer. Proteintech is sponsoring a monthly career development podcast, The Incubator.
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Breast Cancer | 7 Interesting Facts
Learn something you didn't know about breast cancer.
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I Just Want Your Extra Time And Your...KISS-1
Learn about the function of the KISS-1 protein, written by guest blogger Sophie Quick.
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Seeing Clearly: Tips For Staining Cleared Tissue With Antibodies
Improve your immunofluorescence in cleared tissue with expert tips from our guest blogger, Ashley Juavinett.
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7 Tips For Optimizing Your Western Blotting Experiments
How to optimize your western blot protocol
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8 Top Tips For Immunoprecipitation
8 tips on how to perform better IP experiments.
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Mitochondria: Center of Cellular Signaling and Energetic Balance
Mitochondrial markers
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Recalling when and where: How the brain processes episodic memory
Introduction
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10 tips on how to best optimize siRNA transfection
Improve your siRNA transfections with these expert tips.
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Fast Phospho-rward: Making Phosphoprotein Antibodies
A historical perspective on the production of phospo-antibodies.
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Molecular markers for liver cancer
Discover molecular markers for hepatocellular carcinoma.
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Nano Diamonds and Cancer
An introduction to nanoparticle delivery in cancer, written by guest blogger Veronika Benson.
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Can man's best friend help cure cancer
Humans are not the only species on earth that develop cancer; our beloved pets can also succumb to the ‘Big C’ too.
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Can a Bacterial 'Brain' Control a Robot?
A perspective on how technology can be used to control biological systems, by guest blogger Ashley Juavinett
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Making the connection between brain and body
We’re connected, from head to toe...
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Tech Tips | In search of low molecular weight proteins
Learn how to improve your Western blot for low molecular weight proteins.
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