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Publication Spotlight highlights the new and exciting discoveries citing Proteintech antibodies published in scientific journals across the globe.
What matters to Proteintech is the success of its antibodies in the hands of scientists - the people who make the discoveries that change and explain the world around us. In reaching 50,000 citations of Proteintech antibodies, our thanks go out to the talented researchers who made this milestone possible by using Proteintech antibodies in their work.
The molecular causes of many human genetic diseases are yet to be identified. In a recent paper (PMID: 30728324), Wehrle and colleagues show that odontochondrodysplasia (ODCD), a form of skeletal dysplasia, is caused by hypomorphic mutations in the TRIP11 gene. TRIP11 encodes GMAP-210 (Golgi-associated microtubule-binding protein 210) which regulates protein transport and posttranslational modification within the Golgi apparatus. Severely reduced levels of GMAP-210 in patients’ cells disrupt the structure of the Golgi apparatus and abnormal glycosylation of cargo proteins, which negatively impacts chondrocyte differentiation.
The authors investigated posttranslational modifications of protein using western blotting and analyzing band patterns of glycosylated protein species and the resulting shifts of their molecular weights. Immunofluorescence imaging was used to look at the co-localization studies of Golgi proteins and IFT20, a binding partner of GMAP-210 that localizes both to the Golgi and primary cilium.
Proteintech’s rabbit anti-IFT20 antibody (13615-1-AP) was used in this study to show that GMAP-210 protein mediates correct subcellular localization of IFT20 to the Golgi apparatus. This antibody has been validated in western blot, immunohistochemistry, and chromatin immunoprecipitation with genetic knockdown data to demonstrate its specificity.
Catalog number: 13615-1-AP
Tested Applications: WB, IP, IHC, IF, ELISA
IF image: Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 13615-1-AP (IFT20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Reviews: 5 star
Verified customer: "Excellent for IF labelling of the Golgi under PFA fixation."
For more cilia-related antibodies, please see our cilia card.
Additionally, Proteintech’s rabbit anti-decorin antibody (14667-1-AP) was used to look at posttranslational modifications of extracellular matrix components.
The liver plays a crucial role in protein, carbohydrate, and lipid metabolism, the detoxification of drugs, and the breakdown of hormones. Two cell types, hepatocytes and biliary epithelial cells (BECs), are critical to the regenerating capabilities of the liver.
The current issue of Cell Stem Cell (Vol. 25, issue 1) features the work of Pepe-Mooney and coworkers who elucidated the dual role of the YAP signaling pathway in liver cell homeostasis and the initiation of regenerative processes (PMID: 31080134). YAP is a transcription factor that is involved in the regulation of cell proliferation as well as apoptosis. In an elegant assay, the authors utilized FACS followed by a single-cell RNA-seq to compare the expression profiles of 2,344 homeostatic BECs. Upon the differential expression of several YAP target genes, they identified two major cell clusters, YAP activated and non-activated cells. To identify the reason for this heterogeneity in YAP expression, the assay was repeated for BECs and hepatocytes after drug-induced mimicking of chronic liver damage in vivo. The results revealed the importance of YAP expression in the regenerative response of BECs and hepatocytes under liver-damaging conditions. The exposure of BECs to physiological levels of bile acid (BA) revealed the additional role of the YAP signaling pathway in cell homeostasis, dependent on intracellular BA uptake. This study presents a great basis for further investigations to elucidate cell type-dependent roles and the complexity of YAP signaling.
Beside the two major clusters, the initial RNA-seq screening revealed a small subset of cells co-expressing Dmbt1 and Ly6d, which are markers for extrahepatic BECs.
Proteintech’s Rabbit anti-LY6D (Cat# 17361-1-AP) was used for immunofluorescence staining as a differential marker for extrahepatic BECs. Based on the antibody performance it was possible to separate extrahepatic from intrahepatic BECs. This antibody has additionally been validated in Western Blot, immunohistochemistry, and ELISA.
Catalog number: 17361-1-AP
Tested Applications: WB, IHC, ELISA
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17361-1-AP( LY6D Antibody) at dilution of 1:50 (under 40x lens)
Is a child being born to a home with food insecurity? A frigid climate? A place with high toxic exposure? Being able to endow children with genetic adaptations to the environment provides significant evolutionary fitness. The prevailing notion is that these tweaks in the gamete stage are mediated by methylation and histone modifications. In the cover story for July’s Molecular Cell (PMID: 31056445), Jung et al. suggest that transcription factor sites and enhancer loops are also inherited from the parents.
Using a transposon accessibility assay and ChIP-seq, they show that mature sperm have transcription machinery and enhancer loops at many sites, but that the corresponding genes are not actively transcribed until embryogenesis. Comparing the loops of DNA in the parental gametes and embryos, they further showed that many of the CCCTC-binding factor (CTCF) and other transcription factor loops are inherited in a parent-specific manner. Their work introduces chromatin structure and early transcription factor priming as novel parental imprinting mechanisms for further investigation.
Proteintech’s Znf143 antibody (16618-1-AP) was used in this study to show that Zinc-finger transcription factors are present at many of inherited sites. This antibody has been validated in Western blot, immunohistochemistry and chromatin immunoprecipitation with genetic knockdown data to demonstrate its specificity.
Catalog number: 16618-1-AP
Tested Applications: WB, IHC, ChIP
Western blot image:
Mouse brain tissue were subjected SDS PAGE followed by Western blot with 16618-1-AP (Znf143 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.