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Proteintech’s NeutraKine™ neutralizing antibodies are made using HumanKine® human-expressed cytokines and growth factors, so they are made and tested against real human proteins, resulting in superior neutralizing activity and biological effects. They are ideal for pre-clinical and academic studies involving cytokines and growth factors.
Neutralizing antibodies are vital in virology and in investigating cytokines and growth factors. The addition of neutralizing antibodies to cell culture can specifically block the biological activity of their target proteins, allowing for the isolation of a specific protein’s effects.
|Beta-NGF||69009-1-Ig||Mouse Monoclonal||4-20 ng/mL（in the presence of 2 ng/mL HZ-1222）|
|GM-CSF||69003-1-Ig||Mouse Monoclonal||1.5-4 µg/mL （in the presence of 0.2 ng/mL HZ-1002）|
|IL-3||69004-1-Ig||Mouse Monoclonal||0.8-2.4 ng/mL（in the presence of 0.5 ng/mL HZ-1074)|
|IL-4||69005-1-Ig||Mouse Monoclonal||200-500 ng/mL（in the presence of 0.5 ng/mL HZ-1004)|
|IL-6||69001-1-Ig||Mouse Monoclonal||3-15 ng/mL （in the presence of 1 ng/mL HZ-1019）|
|IL-23 p40||69006-1-Ig||Mouse Monoclonal||8-30 ng/mL（in the presence of 4 ng/mL HZ-1254)|
|IFN alpha 2A||69008-1-Ig||Mouse Monoclonal||300-500 ng/mL（in the presence of 100 ng/mL HZ-1066）|
|IFN gamma||69007-1-Ig||Mouse Monoclonal||100-400 ng/mL（in the presence of 5 ng/mL HZ-1301)|
|TNF-alpha||69002-1-Ig||Mouse Monoclonal||10-50 ng/mL (in the presence of 0.05 ng/mL HZ-1014)|
|TGF beta 1||69012-1-Ig||Mouse Monoclonal||
0.5-2 ug/mL (in the presence of 0.03 ng/mL HZ-1011)
|BMP-7||69011-1-Ig||Mouse Monoclonal||1-2 ug/mL (in the presence of 200 ng/mL HZ-1229)|
|IFN beta||69013-1-Ig||Mouse Monoclonal||5-20 ug/mL (in the presence of 10 ng/mL HZ-1298)|
|BMP-4||Mouse Monoclonal||3-10 ug/mL (in the presence of 200 ng/mL HZ-1045)|
|Thrombin Coagulation Factor II||69014-1-Ig||Mouse Monoclonal||5-20 ug/mL (in the presence of 400 ng/mL HZ-3010)|
|Cystatin C||69017-1-Ig||Mouse Monoclonal||0.5 mg/mL (in the presence of 100 ug/mL HZ-1211)|
|IL-10||69018-1-Ig||Mouse Monoclonal||50-200 ng/mL (in the presence of 10 ng/mL HZ-1145)|
|EPO||69019-1-Ig||Mouse Monoclonal||2-5 ug/mL (in the presence of 6 ng/mL HZ-1168)|
|PDGF-BB||69020-1-Ig||Mouse Monoclonal||20-50 ug/mL (in the presence of 20 ng/mL HZ-1308)|
|IL-17A||69021-1-Ig||Mouse Monoclonal||200-600 ng/mL (in the presence of 40ng/mL HZ-1113)|
|FGF-4||69022-1-Ig||Mouse Monoclonal||0.1 -0.4ug/mL (in the presence of 2ng/mL HZ-1218)|
It is common to pre-incubate medium containing a cytokine/growth factor with a neutralizing antibody prior to addition to cells to ensure full neutralization. For best results, we highly recommend Proteintech's HumanKine® human-cell expressed proteins as they are fully compatible with our neutralizing antibodies.
TF-1 human erythroleukemic cells proliferate in response to stimulation by HumanKine® beta NGF (HZ-1222, green line). The stimulation response from HZ-1222 (4ng/mL) is neutralized by increasing concentration of NeutraKine™ beta NGF antibody (69009-1-IG, blue line) and leading competitor’s antibody (brown line). The ND50 of 69009-1-IG is over 100X lower than the leading competitor’s antibody.
|Target||Catalog number||Fold stronger neutralization||Proteintech ND50 (ng/mL)||Leading competitor ND50 (ng/mL)|