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Proteintech’s NeutraKine neutralizing antibodies are made using Humankine® human-expressed cytokines and growth factors, so they are made and tested against real human proteins, resulting in superior neutralizing activity and biological effects. They are ideal for pre-clinical and academic studies involving cytokines and growth factors.
Neutralizing antibodies are vital in virology and in investigating cytokines and growth factors. The addition of neutralizing antibodies to cell culture can specifically block the biological activity of their target proteins, allowing for the isolation of a specific protein’s effects.
|Target||Catalog number||Type||ND50 (ng/mL)|
|IL-23 p40||69006-1-Ig||Mouse Monoclonal||298.3|
|IFN alpha 2A||69008-1-Ig||Mouse Monoclonal||329.4|
|IFN gamma||69007-1-Ig||Mouse Monoclonal||17.3|
It is common to pre-incubate medium containing a cytokine/growth factor with a neutralizing antibody prior to addition to cells to ensure full neutralization. For best results, we highly recommend Proteintech's Humankine human-cell expressed proteins as they are fully compatible with our neutralizing antibodies.
TF-1 human erythroleukemic cells proliferate in response to stimulation by Humankine beta NGF (HZ-1222, green line). The stimulation response from HZ-1222 (4ng/mL) is neutralized by increasing concentration of Neutrakine beta NGF antibody (69009-1-IG, blue line) and leading competitor’s antibody (brown line). The ND50 of 69009-1-IG is over 100X lower than the leading competitor’s antibody.
|Target||Catalog number||Fold stronger neutralization||Proteintech ND50 (ng/mL)||Leading competitor ND50 (ng/mL)|