Nano-Secondary reagents

High resolution and low background

Nano-Secondary® reagents use a novel class of secondary antibodies to provide higher resolution, lower background, and cleaner images. Nano-Secondary reagents have secondary Nanobodies/ VHHs conjugated to Alexa Fluor® fluorescent dyes and bind to primary antibodies with high affinity in a species and subtype specific manner:

  • Faster staining: One-step incubation
  • Higher resolution
  • Low background
  • No cross-reactivity and high subclass specificity

Nano-Secondary reagents can be applied in:

Explore Nano-Secondary reagents
Staining image of secondary nanobody ChromoTek Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488. The anti-mouse IgG1 Nano-Secondary is subclass-specific and does not cross-react with IgGs from other commonly used species (here rabbit) and with mouse IgG2b and IgG3 subclasses.

Multiplexed immunostaining of HeLa cells with 3 subclass-specific alpaca anti-mouse Nano-Secondary reagents. Grey: Mouse IgG1 anti-Vimentin + alpaca anti-mouse IgG1 VHH Alexa Fluor® 647. Green: Mouse IgG2b anti-Lamin + alpaca anti-mouse IgG2b VHH Alexa Fluor® 488. Red: Mouse IgG3 anti-MOT + alpaca anti-mouse IgG3 VHH Alexa Fluor® 568. Scale bar, 10 µm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.