Nano-Secondary reagents
High resolution and low background
Nano-Secondary® reagents use a novel class of secondary antibodies to provide higher resolution, lower background, and cleaner images. Nano-Secondary reagents have secondary Nanobodies/ VHHs conjugated to Alexa Fluor® fluorescent dyes and bind to primary antibodies with high affinity in a species and subtype specific manner:
- Faster staining: One-step incubation
- Higher resolution
- Low background
- No cross-reactivity and high subclass specificity
Nano-Secondary reagents can be applied in:
Explore Nano-Secondary reagents
Multiplexed immunostaining of HeLa cells with 3 subclass-specific alpaca anti-mouse Nano-Secondary reagents. Grey: Mouse IgG1 anti-Vimentin + alpaca anti-mouse IgG1 VHH Alexa Fluor® 647. Green: Mouse IgG2b anti-Lamin + alpaca anti-mouse IgG2b VHH Alexa Fluor® 488. Red: Mouse IgG3 anti-MOT + alpaca anti-mouse IgG3 VHH Alexa Fluor® 568. Scale bar, 10 µm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Articles
Advantages of recombinant Nano-Secondaries
Nano-Secondaries are monoclonal Nanobodies that bind to primary antibodies in a species and subtype specific manner, i.e. Nano-Secondaries...
Important facts to know when using Nano-Secondaries
Nano-Secondaries enable very precise stainings as they bind in a site-specific manner to primary IgG antibodies: they bind either to the Fc or Fab region of the...
The importance of blocking when using Nano-Secondaries for IF
Since Nano-Secondaries lack the Fc part of a conventional antibody, blocking with alpaca or llama serum to minimize binding to Fc receptors is not...
Non visible textHow to choose the best secondary antibody for your application
Secondary antibodies are critical for successful experiments involving antibodies. They provide signal detection and amplification along with extending the...
Non visible textAdvantages of recombinant Nano-Secondaries
Nano-Secondaries are monoclonal Nanobodies that bind to primary antibodies in a species and subtype specific manner, i.e. Nano-Secondaries...
Important facts to know when using Nano-Secondaries
Nano-Secondaries enable very precise stainings as they bind in a site-specific manner to primary IgG antibodies: they bind either to the Fc or Fab region of the...
The importance of blocking when using Nano-Secondaries for IF
Since Nano-Secondaries lack the Fc part of a conventional antibody, blocking with alpaca or llama serum to minimize binding to Fc receptors is not...
Non visible textHow to choose the best secondary antibody for your application
Secondary antibodies are critical for successful experiments involving antibodies. They provide signal detection and amplification along with extending the...
Non visible text