Loading Control Antibodies

The proteins that normally serve as internal controls in your research may not always take center stage, but they are indispensable for conducting meaningful research, most notably for the proper interpretation of western blots.

Loading control proteins provide a reference to evaluate the changes in expression levels of target proteins and help to ensure that any increase or decrease in target protein levels is due to your experimental manipulations, and not due to errors in the western blot procedure.

Proteintech control antibodies are £189 each for a 150ul size vial

View all Loading Control Antibodies

Proteintech vs. other vendors

At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.

We have selected the top cited antibodies from these vendors for you to compare:

Proteintech

Abcam

CST

Sigma-Aldrich

GAPDH Monoclonal antibody Anti-GAPDH antibody [6C5] - Loading Control GAPDH (14C10) Rabbit mAb Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

$189 (150 μl)

$700 (100 μg)

$147 (20 μl)

$327 (100 μl)

$787 (300 μl)

$463 (200 μg)

Catalog Number 60004-1-Ig ab8245 2118 MAB374
Citations 10308 4366 7799 2969
Dilutions WB: 1:50,000-1:500000

IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

IF: 1:200-1:2000

FC: 0.40 ug per 10^6 cells in a 100 µl suspension

WB: 1:500-1:10000

ICC/IF: 1-5 µg/ml

WB: 1:1000

IHC-P: 1:400 - 1:1600

IF-IC: 1:50 - 1:200

FC: 1:100 - 1:400

WB: 1:100 - 1:300

IF/IC 1:100 - 1:300

ELISA: 1:1000000

Applications WB, IP, IF, FC, ELISA WB, ICC/IF WB, IHC-P, IF-IC, FC ELISA, ICC, IF, IHC, IP, WB
Reactivity Human, Mouse, Rat, Yeast, Plant, Zebrafish, Pig, Deer, Goat, Tick, Treeshrew, Cynomorium Songaricum, Chicken, Whiteflies Mouse, Rat, Human Human, Mouse, Rat, Monkey, Bovine, Pig Human, Feline, Pig, Mouse, Rabbit, Fish, Canine, Rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
Hassle-free Refund/Replacement
View Proteintech Guarantee
Host Mouse Mouse Rabbit Mouse
Isotype IgG2b IgG1 IgG1
Clonality Monoclonal Monoclonal Monoclonal Monoclonal
Clone Number 1E6D9 6C5 14C10 6C5
Conjugation Unconjugated Unconjugated Unconjugated Unconjugated
View Proteintech’s GAPDH Monoclonal antibody View Abcam’s Anti-GAPDH antibody [6C5] - Loading Control View CST’s GAPDH (14C10) Rabbit mAb View Sigma-Aldrich’s Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

Proteintech

Abcam

CST

Sigma-Aldrich

Beta Actin Monoclonal antibody Anti-beta Actin antibody β-Actin (13E5) Rabbit mAb Monoclonal Anti-β-Actin antibody produced in mouse

$189 (150 μl)

$595 (50 μg)

$147 (20 μl)

$327 (100 μl)

$787 (300 μl)

$508 (20 μl)

$603 (0.2 ml)

$1,210 (0.5 ml)

Catalog Number 66009-1-Ig ab8227 4970 A5441
Citations 5681 3569 5964 10207
Dilutions WB: 1:20000-1:100000

IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

IHC: 1:20-1:2000

IF: 1:500-1:2000

FC: 0.05 ug per 10^6 cells in a 100 µl suspension

WB: 1/1000 - 1/5000

IHC-P: 0.2 - 1 µg/ml

ICC/IF: 1 µg/ml

WB: 1:1000

IHC-P: 1:50 - 1:200

IF: 1:100 - 1:400

FC: 1:100 - 1:400

IF: 1:1000-1:2000

WB: 1:5000-1:10000

Applications WB, IP, IF, FC, IHC, CoIP, ChIP, ELISA, Cell treatment WB, IHC-P, ICC/IF WB, IHC-P, IF, FC ELISA (i), IF, IHC (p), WB
Reactivity Human, Mouse, Rat, Hamster, Monkey, Pig, Chicken, Rabbit, Zebrafish, Canine, Goat, Arabidopsis, Golden Hamsters, Aedes Albopictus Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Xenopus laevis, Fish, Chinese hamster Human, Mouse, Rat, Monkey, Bovine, Pig Sheep, Carp, Feline, Chicken, Rat, Mouse, Hirudo medicinalis, Rabbit, Canine, Pig, Human, Bovine, Guinea pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
Hassle-free Refund/Replacement
View Proteintech Guarantee
Host Mouse Rabbit Rabbit Mouse
Isotype IgG2b IgG IgG IgG1
Clonality Monoclonal Polyclonal Monoclonal Monoclonal
Clone Number 2D4H5 - 13E5 AC-15
Conjugation Unconjugated Unconjugated Unconjugated Unconjugated
View Proteintech’s Beta Actin Monoclonal antibody View Abcam’s Anti-beta Actin antibody View CST’s β-Actin (13E5) Rabbit mAb View Sigma-Aldrich’s Monoclonal Anti-β-Actin antibody produced in mouse

Last updated: 24th April 2024
Vendors’ pricing may vary slightly

Molecular Weight

Use our table below to help select a loading control for your sample type with a different molecular weight to your protein of interest.

Whole cell/cytoplasmic Nuclear Mitochondrial Serum
Loading control MW (kDa) Loading control MW (kDa) Loading control MW (kDa) Loading control MW (kDa)
Vinculin 117                         
       Transferrin 77
Lamin B1 66                     
HDAC1 65
α-tubulin 52       
β-tubulin 50
β-actin 42
GAPDH 36 TBP (rodent) 33-36
         PCNA 36 VDAC1/Porin 36
TBP (human) 37-43   
   COXIV 19
Histone H3 16