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ADAM5 Polyclonal antibody

ADAM5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22147-1-AP

Synonyms

ADAM5, ADAM5P, TMDCII

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ADAM5 Polyclonal antibody (22147-1-AP)

    WB analysis of HeLa using 22147-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22147-1-AP (ADAM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ADAM5 Polyclonal antibody (22147-1-AP)

    WB analysis of MCF-7 using 22147-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 22147-1-AP (ADAM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ADAM5 Polyclonal antibody (22147-1-AP)

    IHC staining of human testis using 22147-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22147-1-AP (ADAM5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ADAM5 Polyclonal antibody (22147-1-AP)

    IHC staining of human testis using 22147-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22147-1-AP (ADAM5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ADAM5 Polyclonal antibody (22147-1-AP)

    IF Staining of HeLa using 22147-1-AP

    Immunofluorescent analysis of HeLa cells using 22147-1-AP (ADAM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22147-1-AP is ADAM5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM5 fusion protein Ag17590
Full Name ADAM metallopeptidase domain 5 pseudogene
Calculated Molecular Weight 412 aa, 47 kDa
Observed Molecular Weight 36-50 kDa
GenBank Accession NumberBC067864
Gene Symbol ADAM5P
Gene ID (NCBI) 255926
RRIDAB_11182171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM5(putative disintegrin and metalloproteinase domain-containing protein 5) is also named as ADAM5P, TMDC2(tMDCII). The tMDC II, abundantly expressed, sperm surface MDC proteins identified in rodent and macaque systems, is also nonfunctional in the human(PMID:10417343). It has 7 isoforms produced by alternative splicing. It is expressed in testis highly by northern blot. This antibody is specific to ADAM5.

Protocols

Product Specific Protocols
WB protocol for ADAM5 antibody 22147-1-APDownload protocol
IHC protocol for ADAM5 antibody 22147-1-APDownload protocol
IF protocol for ADAM5 antibody 22147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols