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  • Featured Product
  • KD/KO Validated

AGTRAP Polyclonal antibody

AGTRAP Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11559-1-AP

Synonyms

AGTRAP, ATRAP

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using AGTRAP Polyclonal antibody (11559-1-AP)

    IHC staining of human pancreas cancer using 11559-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11559-1-AP (AGTRAP antibody) at dilution of 1:10 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using AGTRAP Polyclonal antibody (11559-1-AP)

    IF Staining of HEK-293 using 11559-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 11559-1-AP (AGTRAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11559-1-AP targets AGTRAP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGTRAP fusion protein Ag2116
Full Name angiotensin II receptor-associated protein
Calculated Molecular Weight 159 aa, 18 kDa
GenBank Accession NumberBC017328
Gene Symbol AGTRAP
Gene ID (NCBI) 57085
RRIDAB_2877778
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for AGTRAP antibody 11559-1-APDownload protocol
IF protocol for AGTRAP antibody 11559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Int J Biol Sci

USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling.

Authors - Dandan Wang
  • KD Validated
View Article
mouseWB,IHC

Diabetes Metab Syndr Obes

Ursolic Acid Treatment Alleviates Diabetic Kidney Injury By Regulating The ARAP1/AT1R Signaling Pathway.

Authors - Tian-Kui Ma
  • KD Validated
View Article
humanWB

Biomed Res Int

PBX3 Promotes Tumor Growth and Angiogenesis via Activation of AT1R/VEGFR2 Pathway in Papillary Thyroid Carcinoma.

Authors - Qin Chen
View Article
human,ratIHC

Cell Death Dis

S-adenosylmethionine upregulates the angiotensin receptor-binding protein ATRAP via the methylation of HuR in NAFLD.

Authors - Tao Guo
View Article