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- KD/KO Validated
ANKRD27 Polyclonal antibody
ANKRD27 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24034-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | A431 cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
The immunogen of 24034-1-AP is ANKRD27 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ANKRD27 fusion protein Ag21239 |
| Full Name | ankyrin repeat domain 27 (VPS9 domain) |
| Calculated Molecular Weight | 1050 aa, 117 kDa |
| Observed Molecular Weight | 117-130 kDa |
| GenBank Accession Number | BC050529 |
| Gene Symbol | ANKRD27 |
| Gene ID (NCBI) | 84079 |
| RRID | AB_2879413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VARP (VPS9-ankyrin-repeat protein, also known as ANKRD27) was originally identified as an N-terminal VPS9 (vacuolar protein sorting 9)-domain-containing protein that possesses guanine nucleotide exchange factor (GEF) activity toward small GTPase Rab21 and contains two ankyrin repeat (ANKR) domains in its central region.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANKRD27 antibody 24034-1-AP | Download protocol |
| IHC protocol for ANKRD27 antibody 24034-1-AP | Download protocol |
| IF protocol for ANKRD27 antibody 24034-1-AP | Download protocol |
| IP protocol for ANKRD27 antibody 24034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy.
| ||
J Cell Sci TI-VAMP/VAMP7-SNARE-Rab-GTPase interaction network within a ciliary membrane targeting complex. | ||
Autophagy RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Francisco (Verified Customer) (04-12-2022) | We have tested the ANKRD27 antibody in Neuro2a, MelJuSo and HeLa cells. While the antibody works in the human cell lines tested, we could not see the same band in the Neuro2a lysates.
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