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ANKRD55 Polyclonal antibody

ANKRD55 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24203-1-AP

Synonyms

ANKRD55, ankyrin repeat domain 55

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ANKRD55 Polyclonal antibody (24203-1-AP)

    WB analysis of HeLa using 24203-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ANKRD55 Polyclonal antibody (24203-1-AP)

    WB analysis of L02 using 24203-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ANKRD55 Polyclonal antibody (24203-1-AP)

    IHC staining of human cervical cancer using 24203-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ANKRD55 Polyclonal antibody (24203-1-AP)

    IHC staining of human cervical cancer using 24203-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANKRD55 Polyclonal antibody (24203-1-AP)

    IF Staining of HeLa using 24203-1-AP

    Immunofluorescent analysis of HeLa cells using 24203-1-AP (ANKRD55 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24203-1-AP is ANKRD55 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD55 fusion protein Ag21271
Full Name ankyrin repeat domain 55
Calculated Molecular Weight 614 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC106914
Gene Symbol ANKRD55
Gene ID (NCBI) 79722
RRIDAB_2879456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD55 also named as Ankyrin repeat domain containing protein 55 is a 614 amino acid protein, which contains 9 ANK repeat. ANKRD55 may exist three alternatively spliced isoforms and belongs to ankyrin family. The function of ANKRD55 is still non-known and need to be further studied.

Protocols

Product Specific Protocols
WB protocol for ANKRD55 antibody 24203-1-APDownload protocol
IHC protocol for ANKRD55 antibody 24203-1-APDownload protocol
IF protocol for ANKRD55 antibody 24203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols