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  • Featured Product
  • KD/KO Validated

ANKRD6 Polyclonal antibody

ANKRD6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24333-1-AP

Synonyms

ANKRD6, ankyrin repeat domain 6, DIVERSIN, KIAA0957

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ANKRD6 Polyclonal antibody (24333-1-AP)

    WB analysis of HEK-293 using 24333-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using ANKRD6 Polyclonal antibody (24333-1-AP)

    WB analysis of fetal human brain using 24333-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ANKRD6 Polyclonal antibody (24333-1-AP)

    IP experiment of HEK-293 using 24333-1-AP

    IP result of anti-ANKRD6 (IP:24333-1-AP, 5ug; Detection:24333-1-AP 1:300) with HEK-293 cells lysate 920ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANKRD6 Polyclonal antibody (24333-1-AP)

    IHC staining of human kidney using 24333-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ANKRD6 Polyclonal antibody (24333-1-AP)

    IHC staining of human ovary using 24333-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANKRD6 Polyclonal antibody (24333-1-AP)

    IHC staining of human kidney using 24333-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ANKRD6 Polyclonal antibody (24333-1-AP)

    IHC staining of human ovary using 24333-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293 cells, fetal human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24333-1-AP targets ANKRD6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD6 fusion protein Ag19328
Full Name ankyrin repeat domain 6
Calculated Molecular Weight 727 aa, 80 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC042173
Gene Symbol ANKRD6
Gene ID (NCBI) 22881
RRIDAB_2879498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway.

Protocols

Product Specific Protocols
WB protocol for ANKRD6 antibody 24333-1-APDownload protocol
IHC protocol for ANKRD6 antibody 24333-1-APDownload protocol
IP protocol for ANKRD6 antibody 24333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Chin J Cancer Res

Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer.

Authors - Rui Bai
View Article
humanWB

Mol Carcinog

Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.

Authors - Kirthana Prabhakar
  • KD Validated
View Article