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ANUBL1 Polyclonal antibody

ANUBL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17478-1-AP

Synonyms

AN1 type zinc finger protein 4, ANUBL1, ZFAND4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANUBL1 Polyclonal antibody (17478-1-AP)

    WB analysis using 17478-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ANUBL1 Polyclonal antibody (17478-1-AP)

    WB analysis using 17478-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ANUBL1 Polyclonal antibody (17478-1-AP)

    IHC staining of human stomach cancer using 17478-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ANUBL1 Polyclonal antibody (17478-1-AP)

    IHC staining of human stomach cancer using 17478-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17478-1-AP is ANUBL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANUBL1 fusion protein Ag11505
Full Name AN1, ubiquitin-like, homolog (Xenopus laevis)
Calculated Molecular Weight 653 aa, 72 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC048968
Gene Symbol ANUBL1
Gene ID (NCBI) 93550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANUBL1 is also named as ZFAND4. The expression of ANUBL1 was specifically upregulated in gastric cancer and it was positively associated with grades of gastric cancer. Moreover, it promoted proliferation of gastric cancer SGC-7901 cells. The mechanism of ANUBL1 upregulation in gastric cancer is that miR-182 suppressed the expression of ANUBL1 through targeting its 3'UTR, and ANUBL1 could downregulate the expression of anti-proliferation-associated miRNAs (miR-148b, miR-375 and miR-182) in SGC-7901 cells. The calculated molecular weight of ANUBL1 is 80 kDa (PMID: 25682742).

Protocols

Product Specific Protocols
WB protocol for ANUBL1 antibody 17478-1-APDownload protocol
IHC protocol for ANUBL1 antibody 17478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols