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Apolipoprotein A II Monoclonal antibody

Apolipoprotein A II Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1A2

Cat no : 66773-1-Ig

Synonyms

Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Apolipoprotein A II Monoclonal antibody (66773-1-Ig)

    WB analysis of human plasma using 66773-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66773-1-Ig (APOA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Apolipoprotein A II Monoclonal antibody (66773-1-Ig)

    IHC staining of human liver cancer using 66773-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Apolipoprotein A II Monoclonal antibody (66773-1-Ig)

    IHC staining of human liver cancer using 66773-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66773-1-Ig targets Apolipoprotein A II in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Apolipoprotein A II fusion protein Ag9863
Full Name apolipoprotein A-II
Calculated Molecular Weight 100 aa, 11 kDa
Observed Molecular Weight 8-11 kDa
GenBank Accession NumberBC005282
Gene Symbol APOA2
Gene ID (NCBI) 336
RRIDAB_2882119
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein A II antibody 66773-1-IgDownload protocol
IHC protocol for Apolipoprotein A II antibody 66773-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols