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APOBEC2 Polyclonal antibody

APOBEC2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20121-1-AP

Synonyms

APOBEC2, ARCD1, ARP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOBEC2 Polyclonal antibody (20121-1-AP)

    WB analysis using 20121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20121-1-AP (APOBEC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IP experiment of mouse skeletal muscle using 20121-1-AP

    IP result of anti-APOBEC2 (IP:20121-1-AP, 4ug; Detection:20121-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IHC staining of mouse heart using 20121-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IHC staining of mouse heart using 20121-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IHC staining of human heart using 20121-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20121-1-AP (APOBEC2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IHC staining of human heart using 20121-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20121-1-AP (APOBEC2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20121-1-AP is APOBEC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOBEC2 fusion protein Ag13699
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC047767
Gene Symbol APOBEC2
Gene ID (NCBI) 10930
RRIDAB_10697687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC2, also named as ARCD1 and ARP1, is a member of the activation-induced deaminase/apolipoprotein B mRNA editing enzyme catalytic polypeptide (APOBEC) cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. APOBEC family members have diverse roles by their ability to edit DNA and/or RNA. Apobec2 is one of the oldest members of the APOBEC family, along with the lymphoid-specific activation-induced deaminase. Unlike other APOBEC family members, the enzymatic activity and substrate of Apobec2 have not been fully demonstrated, and its biologic functions remain unknown. It can be detected as 25-30 kDa.

Protocols

Product Specific Protocols
WB protocol for APOBEC2 antibody 20121-1-APDownload protocol
IHC protocol for APOBEC2 antibody 20121-1-APDownload protocol
IP protocol for APOBEC2 antibody 20121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Oncol

HBV triggers APOBEC2 expression through miR‑122 regulation and affects the proliferation of liver cancer cells.

Authors - Aimei Li
View Article