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ARHGEF9-Specific Polyclonal antibody

ARHGEF9-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20042-1-AP

Synonyms

ARHGEF9, ARHGEF9-Specific, COLLYBISTIN, HPEM 2, KIAA0424, PEM 2, PEM 2 homolog, PEM2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGEF9-Specific Polyclonal antibody (20042-1-AP)

    WB analysis using 20042-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARHGEF9-Specific Polyclonal antibody (20042-1-AP)

    WB analysis of HeLa using 20042-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ARHGEF9-Specific Polyclonal antibody (20042-1-AP)

    WB analysis of human brain using 20042-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20042-1-AP (ARHGEF9-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, human brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20042-1-AP targets ARHGEF9-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Cdc42 guanine nucleotide exchange factor (GEF) 9
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberNM_015185
Gene Symbol ARHGEF9
Gene ID (NCBI) 23229
RRIDAB_10694145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF9, also named as KIAA0424, acts as guanine nucleotide exchange factor (GEF) for CDC42. ARHGEF9 promotes formation of GPHN clusters. This antibody is specific to ARHGEF9.

Protocols

Product Specific Protocols
WB protocol for ARHGEF9-Specific antibody 20042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Synapse

Triptolide injection reduces Alzheimer's disease-like pathology in mice

Authors - Rui Mao
View Article