• Featured Product
  • KD/KO Validated

ATG4D Polyclonal antibody

ATG4D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16924-1-AP

Synonyms

APG4 D, APG4D, ATG4D, AUTL4, Autophagin 4, Cysteine protease ATG4D



Tested Applications

Positive WB detected inhuman brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16924-1-AP targets ATG4D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG4D fusion protein Ag10307
Full Name ATG4 autophagy related 4 homolog D (S. cerevisiae)
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC068992
Gene Symbol ATG4D
Gene ID (NCBI) 84971
RRIDAB_2062024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

Protocols

Product Specific Protocols
WB protocol for ATG4D antibody 16924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma.

Authors - Tianzhi Huang
humanWB

Mol Cell

Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.

Authors - Joanne Durgan
  • KO Validated
humanWB

Cell Rep

Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation

Authors - Rachel Ulferts
  • KD Validated
humanWB

Mol Ther Nucleic Acids

lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation.

Authors - Changhong Liu
humanWB

Autophagy

Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.

Authors - Alexander Agrotis
  • KD Validated