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ATP9A Polyclonal antibody

ATP9A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19504-1-AP

Synonyms

ATP9A, ATPase class II type 9A, ATPase, class II, type 9A, ATPIIA, KIAA0611

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ATP9A Polyclonal antibody (19504-1-AP)

    WB analysis of mouse spleen using 19504-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19504-1-AP (ATP9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of human testis using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of human testis using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of rat brain using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of rat brain using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP9A Polyclonal antibody (19504-1-AP)

    IF Staining of HeLa using 19504-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19504-1-AP (ATP9A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman testis tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19504-1-AP targets ATP9A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ATPase, class II, type 9A
Calculated Molecular Weight 119 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberNM_006045
Gene Symbol ATP9A
Gene ID (NCBI) 10079
RRIDAB_10640806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).

Protocols

Product Specific Protocols
WB protocol for ATP9A antibody 19504-1-APDownload protocol
IHC protocol for ATP9A antibody 19504-1-APDownload protocol
IF protocol for ATP9A antibody 19504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Joleen (Verified Customer) (06-16-2019)

It recognizes ATP9A (top most, prominent band) but also quite a few non-specific ones.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ATP9A Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:19504-1-AP)