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BEGAIN Polyclonal antibody

BEGAIN Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14956-1-AP

Synonyms

BEGAIN, KIAA1446

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BEGAIN Polyclonal antibody (14956-1-AP)

    WB analysis using 14956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14956-1-AP (BEGAIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse brain using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of human pancreas cancer using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse cerebellum using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BEGAIN Polyclonal antibody (14956-1-AP)

    IHC staining of mouse cerebellum using 14956-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, JAR cells, SH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue, mouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14956-1-AP targets BEGAIN in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BEGAIN fusion protein Ag6794
Full Name brain-enriched guanylate kinase-associated homolog (rat)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC002607
Gene Symbol BEGAIN
Gene ID (NCBI) 57596
RRIDAB_2064487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BEGAIN antibody 14956-1-APDownload protocol
IHC protocol for BEGAIN antibody 14956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

The First Nationwide Survey and Genetic Analyses of Bardet-Biedl Syndrome in Japan.

Authors - Makito Hirano
View Article