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  • Featured Product
  • KD/KO Validated

BSAP,PAX5 Polyclonal antibody

BSAP,PAX5 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 26709-1-AP

Synonyms

BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    WB analysis using 26709-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    WB analysis of Raji using 26709-1-AP

    WB result of BSAP,PAX5 antibody (26709-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BSAP,PAX5 transfected Raji cells.

  • Western Blot (WB) analysis of Raji cells using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    WB analysis of Raji using 26709-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    WB analysis of Ramos using 26709-1-AP

    Ramos cells were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Ramos cells using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    IP experiment of Ramos using 26709-1-AP

    IP result of anti-BSAP,PAX5 (IP:26709-1-AP, 4ug; Detection:26709-1-AP 1:1000) with Ramos cells lysate 3600 ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    IHC staining of human lymphoma using 26709-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    IHC staining of human lymphoma using 26709-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    IHC staining of human tonsillitis using 26709-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    IHC staining of human tonsillitis using 26709-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Ramos cells using BSAP,PAX5 Polyclonal antibody (26709-1-AP)

    FC experiment of Ramos using 26709-1-AP

    1X10^6 Ramos cells were intracellularly stained with 0.20 ug/test Anti-Human BSAP,PAX5 (26709-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IP detected inRamos cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 3 publications below

Product Information

26709-1-AP targets BSAP,PAX5 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BSAP,PAX5 fusion protein Ag25147
Full Name paired box 5
Calculated Molecular Weight 391 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC156927
Gene Symbol PAX5
Gene ID (NCBI) 5079
RRIDAB_2880608
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

Protocols

Product Specific Protocols
WB protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
IHC protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
IP protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
FC protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.

Authors - Runzhi Huang
View Article
humanIHC

J Cutan Pathol

Superficial sarcomas with CIC rearrangement are aggressive neoplasms: A series of eight cases.

Authors - Jennifer S Ko
View Article
mouseIHC

Mol Oncol

miR-126 downregulates CXCL12 expression in intestinal epithelial cells to suppress the recruitment and function of macrophages and tumorigenesis in a murine model of colitis-associated colorectal cancer

Authors - Shuai Wu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kenzo (Verified Customer) (03-28-2024)

This antibody was used to detect Pax5 positive cells in tonsil and worked well for immunofluorescence.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Tonsil