BSEP Monoclonal antibody
BSEP Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse , Rat, pig and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3C11D5
Cat no : 67512-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, unboiled HepG2 cells, rat liver tissue, mouse liver tissue, pig lung tissue, HeLa cells, A549 cells |
| Positive IHC detected in | mouse liver tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
| Immunofluorescence (IF) | IF : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
67512-1-Ig targets BSEP in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat, pig samples.
| Tested Reactivity | Human, Mouse , Rat, pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BSEP fusion protein Ag29135 |
| Full Name | ATP-binding cassette, sub-family B (MDR/TAP), member 11 |
| Calculated Molecular Weight | 146 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | NM_003742 |
| Gene Symbol | ABCB11 |
| Gene ID (NCBI) | 8647 |
| RRID | AB_2882733 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage. The calculated MW of ABCB11 is 146 kDa, 67512-1-Ig can detect bands around 150 kDa. It's reported that ABCB11 has several N-glycosylation sites, the observed higher molecular mass may due to posttranslational N-glycosylation. (PMID: 9545351, 24359682)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BSEP antibody 67512-1-Ig | Download protocol |
| IHC protocol for BSEP antibody 67512-1-Ig | Download protocol |
| IF protocol for BSEP antibody 67512-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Vitamin C and vitamin D3 alleviate metabolic-associated fatty liver disease by regulating the gut microbiota and bile acid metabolism via the gut-liver axis | ||
Appl Biochem Biotechnol Sphincter of Oddi Dysfunction Induces Gallstone by Inhibiting the Expression of ABCB11 via PKC-α |
