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BTN3A3 Polyclonal antibody

BTN3A3 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15896-1-AP

Synonyms

BTF3, BTN3A3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using BTN3A3 Polyclonal antibody (15896-1-AP)

    WB analysis of Jurkat using 15896-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15896-1-AP (BTN3A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using BTN3A3 Polyclonal antibody (15896-1-AP)

    WB analysis of U-937 using 15896-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15896-1-AP (BTN3A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using BTN3A3 Polyclonal antibody (15896-1-AP)

    IP experiment of Jurkat using 15896-1-AP

    IP result of anti-BTN3A3 (IP:15896-1-AP, 4ug; Detection:15896-1-AP 1:500) with Jurkat cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BTN3A3 Polyclonal antibody (15896-1-AP)

    IF Staining of HepG2 using 15896-1-AP

    Immunofluorescent analysis of HepG2 cells, using BTN3A3 antibody 15896-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inJurkat cells, U-937 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15896-1-AP targets BTN3A3 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTN3A3 fusion protein Ag8711
Full Name butyrophilin, subfamily 3, member A3
Calculated Molecular Weight 584 aa, 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC015815
Gene Symbol BTN3A3
Gene ID (NCBI) 10384
RRIDAB_2065784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BTN3A3 antibody 15896-1-APDownload protocol
IF protocol for BTN3A3 antibody 15896-1-APDownload protocol
IP protocol for BTN3A3 antibody 15896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols