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C10orf27 Polyclonal antibody

C10orf27 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24424-1-AP

Synonyms

C10orf27, Protein SPATIAL, spatial, TBATA

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using C10orf27 Polyclonal antibody (24424-1-AP)

    WB analysis of HeLa using 24424-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24424-1-AP (C10orf27 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C10orf27 Polyclonal antibody (24424-1-AP)

    WB analysis of HeLa using 24424-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24424-1-AP (C10orf27 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C10orf27 Polyclonal antibody (24424-1-AP)

    IHC staining of mouse brain using 24424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24424-1-AP (C10orf27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C10orf27 Polyclonal antibody (24424-1-AP)

    IHC staining of human lung cancer using 24424-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24424-1-AP (C10orf27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C10orf27 Polyclonal antibody (24424-1-AP)

    IHC staining of human lung cancer using 24424-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24424-1-AP (C10orf27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C10orf27 Polyclonal antibody (24424-1-AP)

    IHC staining of mouse brain using 24424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24424-1-AP (C10orf27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24424-1-AP is C10orf27 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C10orf27 fusion protein Ag19625
Full Name chromosome 10 open reading frame 27
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC137543
Gene Symbol C10orf27
Gene ID (NCBI) 219793
RRIDAB_2879542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C10orf27, also named as TBATA and SPATIAL, may play a role in spermatid differentiation. We always got 40-45 kDa band in our detection.

Protocols

Product Specific Protocols
WB protocol for C10orf27 antibody 24424-1-APDownload protocol
IHC protocol for C10orf27 antibody 24424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols