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C10orf67 Polyclonal antibody

C10orf67 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24459-1-AP

Synonyms

C10orf67, RP11 792P23.2

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using C10orf67 Polyclonal antibody (24459-1-AP)

    WB analysis of PC-3 using 24459-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24459-1-AP (C10orf67 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using C10orf67 Polyclonal antibody (24459-1-AP)

    WB analysis of human testis using 24459-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24459-1-AP (C10orf67 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using C10orf67 Polyclonal antibody (24459-1-AP)

    WB analysis of A549 using 24459-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24459-1-AP (C10orf67 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPC-3 cells, A549 cells, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24459-1-AP is C10orf67 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C10orf67 fusion protein Ag21465
Full Name chromosome 10 open reading frame 67
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC035732
Gene Symbol C10orf67
Gene ID (NCBI) 256815
RRIDAB_2879558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF17 also termed as ring finger protein 17 is a 1623 amino acid protein, which contains 1 ring type zinc finger and 4 tudor domains. RNF17 is specifically expressed in testis. The RING finger motif presents in many ubiquitin E3 ligases. RNF17 interacts with all four members of the Mad family (Mad1, Mxi1, Mad3 and Mad4), which are basic-helix-loop-helix-leucine zipper transcription factors of the Myc oncoprotein network. RNF17 is component of the tectonic-like complex, which is required for ciliogenesis and sonic hedgehog/SHH signaling. RNF17 is able to form dimers or polymers both in vitro and in vivo, indicating that it may play a role in the assembly of RNF17 granules. RNF17 encodes a novel key regulator of spermiogenesis.

Protocols

Product Specific Protocols
WB protocol for C10orf67 antibody 24459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols