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C11orf74 Polyclonal antibody

C11orf74 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25758-1-AP

Synonyms

C11orf74, Protein HEPIS

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using C11orf74 Polyclonal antibody (25758-1-AP)

    WB analysis of mouse skeletal muscle using 25758-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25758-1-AP (C11orf74 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C11orf74 Polyclonal antibody (25758-1-AP)

    IHC staining of human liver cancer using 25758-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25758-1-AP (C11orf74 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C11orf74 Polyclonal antibody (25758-1-AP)

    IHC staining of human liver cancer using 25758-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25758-1-AP (C11orf74 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25758-1-AP is C11orf74 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C11orf74 fusion protein Ag22770
Full Name chromosome 11 open reading frame 74
Calculated Molecular Weight 221 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC051031
Gene Symbol C11orf74
Gene ID (NCBI) 119710
RRIDAB_2880227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C11orf74, also named as Protein HEPIS, has two isoforms with MW 25 kDa and 17 kDa.

Protocols

Product Specific Protocols
WB protocol for C11orf74 antibody 25758-1-APDownload protocol
IHC protocol for C11orf74 antibody 25758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols