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Vertnin Polyclonal antibody

Vertnin Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24824-1-AP

Synonyms

C14orf115, Vertnin, VRTN

Validation Data Gallery

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  • Western Blot (WB) analysis of SGC-7901 cells using Vertnin Polyclonal antibody (24824-1-AP)

    WB analysis of SGC-7901 using 24824-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24824-1-AP (Vertnin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Vertnin Polyclonal antibody (24824-1-AP)

    IHC staining of human placenta using 24824-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24824-1-AP (Vertnin antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Vertnin Polyclonal antibody (24824-1-AP)

    IHC staining of human placenta using 24824-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24824-1-AP (Vertnin antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vertnin Polyclonal antibody (24824-1-AP)

    IF Staining of HepG2 using 24824-1-AP

    Immunofluorescent analysis of HepG2 cells using 24824-1-AP (Vertnin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24824-1-AP is Vertnin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vertnin fusion protein Ag20678
Full Name chromosome 14 open reading frame 115
Calculated Molecular Weight 702 aa, 78 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC053325
Gene Symbol C14orf115
Gene ID (NCBI) 55237
RRIDAB_2879745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vertnin, also named as VRTN and C14orf115, belongs to the vertnin family. It is an essential factor for development of the embryo in a wide range of organisms.

Protocols

Product Specific Protocols
WB protocol for Vertnin antibody 24824-1-APDownload protocol
IHC protocol for Vertnin antibody 24824-1-APDownload protocol
IF protocol for Vertnin antibody 24824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols