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C15orf55 Polyclonal antibody

C15orf55 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28639-1-AP

Synonyms

C15orf55, DKFZp434O192, Nuclear protein in testis, NUT, Protein NUT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C15orf55 Polyclonal antibody (28639-1-AP)

    WB analysis using 28639-1-AP

    Mouse liver tissue were subjected to SDS PAGE followed by western blot with 28639-1-AP (C15orf55 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse testis using 28639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse skin using 28639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse skin using 28639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse skin using 28639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C15orf55 Polyclonal antibody (28639-1-AP)

    IHC staining of mouse testis using 28639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C15orf55 Polyclonal antibody (28639-1-AP)

    IF Staining of A431 using 28639-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C15orf55 antibody (28639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse testis tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28639-1-AP targets C15orf55 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C15orf55 fusion protein Ag29239
Full Name chromosome 15 open reading frame 55
Calculated Molecular Weight 1132 aa, 120 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC114567
Gene Symbol C15orf55
Gene ID (NCBI) 256646
RRIDAB_2881186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C15orf55 antibody 28639-1-APDownload protocol
IHC protocol for C15orf55 antibody 28639-1-APDownload protocol
IF protocol for C15orf55 antibody 28639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols