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- KD/KO Validated
COSMC Polyclonal antibody
COSMC Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 19254-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Caco-2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 19254-1-AP is COSMC Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COSMC fusion protein Ag6899 |
| Full Name | C1GALT1-specific chaperone 1 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36-37 kDa |
| GenBank Accession Number | BC050441 |
| Gene Symbol | C1GALT1C1 |
| Gene ID (NCBI) | 29071 |
| RRID | AB_10638003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COSMC (C1GALT1C1) is a molecular chaperone required for expression of active T-synthase (CIGALT1) which catalyzes the synthesis of Tn antigen. Tn antigens are cancer-associated glycans and play an important role in tumor progression. Dysfunctional COSMC is able to convert a wild type protein into a tumor-specific antigen affecting tumor cell biology. COSMC overexpression had been observed in various cancers. COSMC mutations have been observed in an autoimmune disease Tn syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COSMC antibody 19254-1-AP | Download protocol |
| IHC protocol for COSMC antibody 19254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging Cell Elevated miR-124-3p in the aging colon disrupts mucus barrier and increases susceptibility to colitis by targeting T-synthase. | ||
Aging (Albany NY) Cosmc transfection decreases malignant behavior of Tn + cells and enhances sensitivity to apoptosis when induced by Apo2L/TRAIL via alteration of O-glycan structure | ||
Mol Cancer COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer. | ||
Biochim Biophys Acta Mol Cell Res SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization | ||
Respir Physiol Neurobiol The Cosmc-mediated effects of neutrophil elastase on T antigen expression in BEAS-2B cells. | ||
Foods Si-Wu Water Extracts Protect against Colonic Mucus Barrier Damage by Regulating Muc2 Mucin Expression in Mice Fed a High-Fat Diet |
