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C1orf83 Polyclonal antibody

C1orf83 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20940-1-AP

Synonyms

C1orf83, RP4 758J24.3, TCEANC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1orf83 Polyclonal antibody (20940-1-AP)

    WB analysis using 20940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20940-1-AP (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of human brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of human brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of mouse brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of mouse brain using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of mouse cerebellum using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of mouse cerebellum using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of human breast cancer using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C1orf83 Polyclonal antibody (20940-1-AP)

    IHC staining of human breast cancer using 20940-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C1orf83 Polyclonal antibody (20940-1-AP)

    IF Staining of HeLa using 20940-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman brain tissue, human breast cancer tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20940-1-AP targets C1orf83 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf83 fusion protein Ag15040
Full Name chromosome 1 open reading frame 83
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC035374
Gene Symbol C1orf83
Gene ID (NCBI) 127428
RRIDAB_10860108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C1orf83 antibody 20940-1-APDownload protocol
IHC protocol for C1orf83 antibody 20940-1-APDownload protocol
IF protocol for C1orf83 antibody 20940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols