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  • Featured Product
  • KD/KO Validated

ATAT1 Polyclonal antibody

ATAT1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 28828-1-AP

Synonyms

MEC17, Alpha-TAT, ATAT1, C6orf134, DKFZp547J097, FLJ13158, Nbla00487, TAT, UPF0635 protein C6orf134

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATAT1 Polyclonal antibody (28828-1-AP)

    WB analysis using 28828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATAT1 Polyclonal antibody (28828-1-AP)

    WB analysis using 28828-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of rat testis using 28828-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of rat testis using 28828-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of mouse testis using 28828-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C6orf134 Polyclonal antibody (28828-1-AP)

    IHC staining of mouse testis using 28828-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    FC experiment of A431 using 28828-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ATAT1 (28828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, U-251 cells, THP-1 cells, U-87 MG cells
Positive IHC detected inrat testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28828-1-AP targets ATAT1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATAT1 fusion protein Ag28757
Full Name chromosome 6 open reading frame 134
Calculated Molecular Weight 47 kDa
Observed Molecular Weight42-45 kDa
GenBank Accession NumberNM_001031722
Gene Symbol C6orf134
Gene ID (NCBI) 79969
RRIDAB_2881219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.

Protocols

Product Specific Protocols
WB protocol for ATAT1 antibody 28828-1-APDownload protocol
IHC protocol for ATAT1 antibody 28828-1-APDownload protocol
IF protocol for ATAT1 antibody 28828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

MedComm (2020)

Matrix stiffness-induced α-tubulin acetylation is required for skin fibrosis formation through activation of Yes-associated protein

Authors - Dongsheng Wen
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

K. (Verified Customer) (10-26-2023)

Each well had 20 ug of protein extract added. On a Nitrocellulose Blotting Membrane, transfer was carried out for two hours at 400 mA at 4 oC. Membrane blocking was carried out at room temperature with 5% BSA in TBS-Tween20 under light shaking. Antibodies were diluted in PBS-Tween20 containing 5% non-fat milk. -The primary antibody incubations were carried out as follows: 1:1000 for three hours at room temperature.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human and mouse Liver cells