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CCZ1 Polyclonal antibody

CCZ1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22159-1-AP

Synonyms

C7orf28A, C7orf28B, CCZ1, CGI 43, H_DJ1163J12.2, UPF0550 protein C7orf28

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CCZ1 Polyclonal antibody (22159-1-AP)

    WB analysis of NIH/3T3 using 22159-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22159-1-AP (CCZ1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using CCZ1 Polyclonal antibody (22159-1-AP)

    IP experiment of NIH/3T3 using 22159-1-AP

    IP result of anti-CCZ1 (IP:22159-1-AP, 4ug; Detection:22159-1-AP 1:500) with NIH/3T3 cells lysate 1500ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CCZ1 Polyclonal antibody (22159-1-AP)

    IHC staining of mouse liver using 22159-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22159-1-AP (CCZ1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CCZ1 Polyclonal antibody (22159-1-AP)

    IHC staining of mouse liver using 22159-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22159-1-AP (CCZ1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22159-1-AP is CCZ1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCZ1 fusion protein Ag17804
Full Name chromosome 7 open reading frame 28A
Calculated Molecular Weight 482 aa, 56 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC132749
Gene Symbol C7orf28A
Gene ID (NCBI) 51622
RRIDAB_2879011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCZ1 antibody 22159-1-APDownload protocol
IHC protocol for CCZ1 antibody 22159-1-APDownload protocol
IP protocol for CCZ1 antibody 22159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH.

Authors - Hanhai Zeng
View Article