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C7orf43 Polyclonal antibody

C7orf43 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27790-1-AP

Synonyms

C7orf43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C7orf43 Polyclonal antibody (27790-1-AP)

    WB analysis using 27790-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27790-1-AP (C7orf43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using C7orf43 Polyclonal antibody (27790-1-AP)

    IHC staining of human liver using 27790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using C7orf43 Polyclonal antibody (27790-1-AP)

    IHC staining of human liver using 27790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27790-1-AP targets C7orf43 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C7orf43 fusion protein Ag27149
Full Name chromosome 7 open reading frame 43
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC015722
Gene Symbol C7orf43
Gene ID (NCBI) 55262
RRIDAB_2880972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The predicted MW of C7orf43 is 62 kDa. Catalog#27790-1-AP recognises 65-70 kDa band may due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for C7orf43 antibody 27790-1-APDownload protocol
IHC protocol for C7orf43 antibody 27790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols